CD63 Protein, Human, Recombinant (His)

CD63 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 13 kDa and the accession number is P08962-1.

CD63 Protein, Human, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
100 μg	在庫あり	￥ 88,500
200 μg	約5 days	￥ 156,500
500 μg	約5 days	￥ 330,500

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生物学的情報	Testing in progress
説明	CD63 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 13 kDa and the accession number is P08962-1.

Species	Human
Expression Host	HEK293 Cells
Tag	His
Accession Number	P08962-1
別名	OMA81H, TSPAN30, LAMP-3, MLA1, ME491, CD63 molecule
Construction	The second extracellular domain of Human CD63 (P08962-1) (Ala 103-Val 203) was fused with a polyhistidine tag at the C-terminus and a signal peptide at the N-terminus.
Protein Purity	> 95 % as determined by SDS-PAGE
分子量	13 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cluster of differentiation 63 (CD63) is a member of the CD family and the transmembrane 4 superfamily, also known as the tetraspanin family. CD63 is a cell surface glycoprotein characterized by the presence of four hydrophobic domains. CD63 had functions in mediating signal transduction processes and then regulate a variety of cellular processes such as cell proliferation, activation and motility. It has been reported that CD63 protein associated with tumor progression and served as a blood platelet activation marker and the deficiency of this protein may be associated with Hermansky-Pudlak syndrome.

参考文献

1. Zola H,et al.(2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5. 2. Ho IC,et al.(2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35. 3. Matesanz-Isabel J,et al.(2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12. 4. Radford KJ,et al.(1996) Associates with Transmembrane 4 Superfamily Members, CD9 and CD81, and with beta 1 Integrins in Human Melanoma. Biochemical Biophysical Research Communications. 222(1): 13-18. 5. Metzelaar MJ,et al.(1991) CD63 antigen, A novel lysosomal membrane glycoprotein, cloned by a screening procedure for intracellular antigens in eukaryotic cells. The journal of biological chemistry. 266: 3239-45.

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CD63 Protein, Human, Recombinant (His)

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