CD64 Protein, Human, Recombinant (His)

CD64 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 32 kDa and the accession number is P12314-1.

CD64 Protein, Human, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
100 μg	在庫あり	￥ 65,000
200 μg	約5 days	￥ 114,000
500 μg	約5 days	￥ 243,500

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生物学的情報

1. Immobilized Anti-CD20(Ro) Antibody (Rituximab) at 1 μg/mL (100 μL/well) can bind Recombinant Human CD64/FCGR1A Protein (His Tag), the EC50 is 0.6-2.0 ng/mL.
2. Captured Human FcγRI/CD64 recombinant protein on Anti-His Chip can bind Bevacizumab (IgG1) with an affinity constant of 5.5 nM as determined in an SPR assay.
3. Captured Human FcγRI/CD64 recombinant protein on Anti-His Chip can bind Nivolumab (IgG4) with an affinity constant of 13.6 nM as determined in an SPR assay.
4. Loaded Human FcγRI / CD64 recombinant protein on His1K Biosensor, can bind Nivolumab (IgG4) with an affinity constant of 8.5 nM as determined in a BLI assay.
5. Loaded Human FcγRI / CD64 recombinant protein on His1K Biosensor, can bind Bevacizumab (IgG1) with an affinity constant of 4.7 nM as determined in a BLI assay.
6. Loaded Rituximab (IgG1) on ProA Biosensor, can bind Human FcγRI/CD64 recombinant protein with an affinity constant of 0.38 nM as determined in a BLI assay.
7. The purity of Human CD64/FCGR1A Protein (His Tag) is more than 95% and the molecular weight of this protein is around 38-52 kDa verified by SEC-MALS.

説明

CD64 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 32 kDa and the accession number is P12314-1.

Species	Human
Expression Host	HEK293 Cells
Tag	His
Accession Number	P12314-1
別名	Fc γ RI, Fc receptor, IgG, high affinity I, Fc gamma RI, CD64
Construction	The extracellular domain of Human CD64A (NP_000557.1) (Met 1-Pro 288) was expressed, fused with a polyhistidine tag at the C-terminus.
Protein Purity	> 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC
分子量	32 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	High affinity immunoglobulin gamma Fc receptor I, also known as FCGR1 and CD64, is an integral membraneglycoprotein and a member of the immunoglobulin superfamily. CD64 is a high affinity receptor for the Fc region of IgG gamma and functions in both innate and adaptive immune responses. Receptors that recognize the Fc portion of IgG function in the regulation of immune response and are divided into three classes designated CD64, CD32, and CD16. CD64 is structurally composed of asignal peptidethat allows its transport to the surface of a cell, threeextracellularimmunoglobulin domainsof the C2-type that it uses to bind antibody, a hydrophobictransmembrane domain, and a short cytoplasmic tail. CD64 isconstitutivelyfound on only macrophages and monocytes, but treatment of polymorphonuclear leukocyteswith cytokines likeIFNγandG-CSFcan induce CD64 expression on these cells. The inactivation of the mouse CD64 resulted in a wide range of defects in antibody Fc-dependent functions. Mouse CD64 is an early participant in Fc-dependent cell activation and in the development of immune responses.

参考文献

1. Allen J.M., et al.,(1988), Nucleotide sequence of three cDNAs for the human high affinity Fc receptor (FcRI). Nucleic Acids Res. 16:11824-11824. 2. Allen J.M., et al., (1989), Isolation and expression of functional high-affinity Fc receptor complementary DNAs.Science 243:378-381. 3. Porges A.J., et al.,(1992), Novel Fc gamma receptor I family gene products in human mononuclear cells.J. Clin. Invest. 90:2102-2109.

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CD64 Protein, Human, Recombinant (His)

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