CD73 Protein, Rat, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 59.4 kDa and the accession number is Q66HL0.
パッケージサイズ | 在庫状況 | 単価(税別) |
---|---|---|
50 μg | 在庫あり | ¥ 103,500 |
100 μg | 約5 days | ¥ 177,000 |
200 μg | 約5 days | ¥ 301,000 |
500 μg | 約5 days | ¥ 610,500 |
生物学的情報 | Measured by its ability to hydrolyze the 5’-phosphate group from the substrate adenosine-5’-monophosphate (AMP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit . The specific activity is >15,000 pmol/min/µg. |
説明 | CD73 Protein, Rat, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 59.4 kDa and the accession number is Q66HL0. |
Species | Rat |
Expression Host | HEK293 Cells |
Tag | C-His |
Accession Number | Q66HL0 |
別名 | 5'-nucleotidase, ecto (CD73) |
Construction | The Rat NT5E(Q66HL0) (Met1-Lys549) was expressed with a polyhistidine tag at the C-terminus. |
Protein Purity |
> 90 % as determined by SDS-PAGE
|
分子量 | 59.4 kDa (predicted) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing Tris, NaCl and CaCl2, pH 7.5, with Glycerol as protectants. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping |
In general, Lyophilized powders are shipping with blue ice. |
Research Background | 5'-nucleotidase, also known as NT5E, NTE, and CD73, is a cell membrane protein that belongs to the 5'-nucleotidase family. CD73 is a glycosylphosphatidylinositol (GPI) anchored purine salvage enzyme expressed on the surface of human T and B lymphocytes. CD73 catalyzes the conversion of purine and pyrimidine ribo- and deoxyribonucleoside monophosphates to the corresponding nucleosides. CD73 serves as a costimulatory molecule in activating T cells. CD73 generated adenosine functions in cell signaling in many physiologic systems, including intestinal epithelium, ischemic myocardium, and cholinergic synapses. CD73 might mediate lymphocyte-stromal cell interactions or condition the local microenvironment to facilitate lymphocyte development and/or function. In CD73-depleted cells, surface levels of the leukocyte adhesion molecules ICAM-1, VCAM-1, and E-selectin increase. CD73 produces extracellular adenosine, which then acts on G protein-coupled purinergic receptors to induce cellular responses. CD73 has also been reported to regulate the expression of pro-inflammatory molecules in mouse endothelium. |
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Please read the User Guide of Recombinant Proteins for more specific information.
CD73 Protein, Rat, Recombinant (His) 5'-nucleotidase, ecto CD73 5'-nucleotidase, ecto (CD73) CD-73 CD 73 recombinant recombinant-proteins proteins protein