CD79B Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with C-Fc tag. The predicted molecular weight is 45-65 KDa and the accession number is P40259.
| 生物学的情報 |
Loaded Human CD79B-Fc on APS Biosensor, can bind Anti-Human CD79B mAb-Fc with an affinity constant of 0.4 nM as determined in BLI assay. (Regularly tested)
|
| 説明 |
CD79B Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with C-Fc tag. The predicted molecular weight is 45-65 KDa and the accession number is P40259. |
| Species |
Human
|
| Expression Host |
Human Cells
|
| Tag |
C-Fc
|
| Accession Number |
P40259
|
| 別名 |
Immunoglobulin-Associated B29 Protein, B-Cell-Specific Glycoprotein B29, B-Cell Antigen Receptor Complex-Associated Protein Beta Chain, IGB, B-Cell Antigen Receptor Complex-Associated Protein β Chain, Ig-β, B29, CD79b, Ig-Beta
|
| Amino Acid |
Ala29-Asp159
|
| Construction |
Recombinant Human CD79B is produced by our Mammalian expression system and the target gene encoding Ala29-Asp159 is expressed with a human IgG1 Fc tag at the C-terminus.
|
| Protein Purity |
Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
|
| 分子量 |
45-65 KDa, reducing conditions
|
| Endotoxin |
Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
|
| Formulation |
Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
|
| Reconstitution |
Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
|
| Stability & Storage |
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
|
| Shipping |
The product is shipped at ambient temperature.
Upon receipt, store it immediately at the temperature listed below.
|
| Research Background |
CD79B is a single-pass type I membrane protein. CD79B contains one Ig-like V-type domain and one ITAM domain. CD79B is required in cooperation with CD79A for initiation of the signal transduction cascade activated by the B-cell antigen receptor complex (BCR), which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. CD79B enhances phosphorylation of CD79A, possibly by recruiting kinases that phosphorylate CD79A or by recruiting proteins that bind to CD79A and protect it from dephosphorylation.
|
Calculator
Reconstitution Calculator
Recombinant Proteins Dilute Calculator
Specific Activity Calculator
bottom
