CD8A & CD8B Heterodimer Protein, Human, Recombinant (Flag & His) is expressed in HEK293 mammalian cells with Flag and His tag. The predicted molecular weight is 44.1 kDa and the accession number is NP_001759.3&NP_004922.1.
| 生物学的情報 |
Testing in progress
|
| 説明 |
CD8A & CD8B Heterodimer Protein, Human, Recombinant (Flag & His) is expressed in HEK293 mammalian cells with Flag and His tag. The predicted molecular weight is 44.1 kDa and the accession number is NP_001759.3&NP_004922.1. |
| Species |
Human
|
| Expression Host |
HEK293 Cells
|
| Tag |
C-Flag, C-His
|
| Accession Number |
NP_001759.3&NP_004922.1
|
| Construction |
The Human CD8A (NP_001759.3) (Met1-Asp182) was fused with a flag tag at the C-terminus, constructed the plasmid 1; The Human CD8B (NP_004922.1) (Met1-Pro170) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the Human CD8A&CD8B heterodimer was purified.
|
| Protein Purity |
> 95 % as determined by SDS-PAGE
|
| 分子量 |
44.1 kDa (predicted)
|
| Endotoxin |
< 1.0 EU/μg of the protein as determined by the LAL method.
|
| Formulation |
Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
|
| Reconstitution |
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
|
| Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
|
| Shipping |
In general, Lyophilized powders are shipping with blue ice.
|
Calculator
Reconstitution Calculator
Recombinant Proteins Dilute Calculator
Specific Activity Calculator
bottom
