CD98 Protein, Human, Recombinant (His)

CD98 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 49.2 kDa and the accession number is P08195-1.

CD98 Protein, Human, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
100 μg	在庫あり	￥ 103,500
200 μg	約5 days	￥ 175,000
500 μg	約5 days	￥ 346,500

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生物学的情報	Testing in progress
説明	CD98 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 49.2 kDa and the accession number is P08195-1.

Species	Human
Expression Host	HEK293 Cells
Tag	His
Accession Number	P08195-1
別名	4T2HC, 4F2, CD98HC, NACAE, MDU1, 4F2HC, CD98, solute carrier family 3 (amino acid transporter heavy chain), member 2
Construction	The Human SLC3A2 isoform 1 (P08195-1) (Arg 206-Ala 630) was expressed, with a polyhistidine tag at the N-terminus.
Protein Purity	> 97 % as determined by SDS-PAGE
分子量	49.2 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	4F2 cell-surface antigen heavy chain, also known as 4F2 heavy chain antigen, Lymphocyte activation antigen 4F2 large subunit, CD98, SLC3A2 and MDU1, is a single-pass type I I membrane protein that belongs to the SLC3A transporter family. SLC3A2 / MDU1 is expressed ubiquitously in all tissues tested with highest levels detected in kidney, placenta and testis and weakest level in thymus. During gestation, expression in the placenta is significantly stronger at full-term than at the mid-trimester stage. SLC3A2 / MDU1 is expressed in HUVECS and at low levels in resting peripheral blood T-lymphocytes and quiescent fibroblasts. It is expressed in fetal liver and in the astrocytic process of primary astrocytic gliomas. SLC3A2 / MDU1 is also expressed in retinal endothelial cells and in the intestinal epithelial cell line Caco2-BBE. SLC3A2 / MDU1 is required for the function of light chain amino-acid transporters. It is involved in sodium-independent, high-affinity transport of large neutral amino acids such as phenylalanine, tyrosine, leucine, arginine and tryptophan. SLC3A2 / MDU1 is involved in guiding and targeting of LAT1 and LAT2 to the plasma membrane. When associated with SLC7A6 or SLC7A7, SLC3A2 / MDU1 acts as an arginine/glutamine exchanger, following an antiport mechanism for amino acid transport, influencing arginine release in exchange for extracellular amino acids. SLC3A2 / MDU1 plays a role in nitric oxide synthesis in human umbilical vein endothelial cells (HUVECs) via transport of L-arginine. It is required for normal and neoplastic cell growth. When associated with SLC7A5/LAT1, SLC3A2 / MDU1 is also involved in the transport of L-DOPA across the blood-brain barrier, and that of thyroid hormones triiodothyronine (T3) and thyroxine (T4) across the cell membrane in tissues such as placenta.

参考文献

1. Torrents D. et al., 1998, J Biol Chem. 273: 32437-45. 2. Pfeiffer R. et al., 1999, EMBO J. 18: 49-57. 3. Broeer A. et al., 2000, Biochem J. 349: 787-95. 4. Yanagida O. et al., 2001, Biochim Biophys Acta. 1514: 291-302. 5. Broeer A. et al., 2001, Biochem J. 355: 725-31. 6. Fort J. et al., 2007, J Biol Chem. 282: 31444-52.Mayya V. et al., 2009, Sci Signal. 2: RA46-RA46.

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CD98 Protein, Human, Recombinant (His)

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