CES1 Protein, Human, Recombinant (His)

CES1 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 62.08 kDa and the accession number is NP_001020365.

CES1 Protein, Human, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
50 μg	約5 days	￥ 100,500
500 μg	約5 days	￥ 897,500

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説明	CES1 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 62.08 kDa and the accession number is NP_001020365.

Species	Human
Expression Host	HEK293 Cells
Tag	C-His
Accession Number	NP_001020365
別名	PCE-1, hCE-1, carboxylesterase 1, CE-1, TGH, HMSE, HMSE1, SES1, CEH, ACAT, CES2, REH
Construction	The Human CES1 (NP_001020365) (Met1-Leu567) was expressed with a polyhistidine tag at the C-terminus.
Protein Purity	≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
分子量	62.08 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	CES1 encodes carboxylesterase-1, an important drug-metabolizing enzyme with high expression in the liver. Variants generated by the exchange of segments with CES1P1 complicate the genotyping of CES1. CES1 is involved in the hydrolysis of ester group-containing xenobiotic and endobiotic compounds including several essential and commonly used drugs. The individual variation in the efficacy and tolerability of many drugs metabolized by CES1 is considerable. However, it has been problematic to express recombinant CES1 in bacterial expression systems due to low solubility, with the CES1 protein being mainly expressed in inclusion bodies, accompanied by insufficient purity issues. CES1 is capable of catalyzing the hydrolysis of a wide range of therapeutic agents, toxins, and endogenous compounds. Accumulating studies have demonstrated associations between the expression and activity of CES1 and the pharmacokinetics and/or pharmacodynamics of CES1 substrate medications.

参考文献

1. Sanford JC,et al.(2016) Regulatory effects of genomic translocations at the human carboxylesterase-1 (ces1) gene locus. Pharmacogenet Genomics 26 (5): 197-207. 2. Rasmussen HB,et al.(2015) Individualization of treatments with drugs metabolized by ces1: Combining genetics and metabolomics. Pharmacogenomics 16 (6): 649-665. 3. Qian Y,et al.(2020) Natural products as modulators of ces1 activity. Drug Metab Dispos 48 (10): 993-1007. 4. Ferrero-Miliani L,et al.(2017) Reappraisal of the genetic diversity and pharmacogenetic assessment of ces1. Pharmacogenomics 18 (13): 1241-1257.

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CES1 Protein, Human, Recombinant (His)

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