CK2 alpha/CSNK2A1 Protein, Human, Recombinant (GST) is expressed in Baculovirus insect cells with GST tag. The predicted molecular weight is 71.4 kDa and the accession number is P68400-1.
説明 | CK2 alpha/CSNK2A1 Protein, Human, Recombinant (GST) is expressed in Baculovirus insect cells with GST tag. The predicted molecular weight is 71.4 kDa and the accession number is P68400-1. |
Species | Human |
Expression Host | Baculovirus Insect Cells |
Tag | GST |
Accession Number | P68400-1 |
別名 | CK2 α/CSNK2A1, CKII, CSNK2A3, CK2A1, casein kinase 2, α1 polypeptide, casein kinase 2, alpha 1 polypeptide |
Construction | The Human CSNK2A1 isoform 1 (NP_808227.1) (Met 1-Gln 391) was fused with the GST tag at the N-terminus. |
Protein Purity | > 90 % as determined by SDS-PAGE |
分子量 | 71.4 kDa (predicted) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Supplied as sterile 50 mM Tris, 100 mM NaCl, 0.5 mM PMSF, 0.5 mM GSH, pH 8.0. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage |
It is recommended to store the product under sterile conditions at -20℃ to -80℃. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping |
Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice. |
Research Background | Casein kinase II subunit alpha, also known as CK II alpha, CSNK2A1 and CK2A1, is a member of the protein kinase superfamily, Ser / Thr protein kinase family and CK2 subfamily. Casein kinase II (CSNK2A1) is a serine / threonine protein kinase that phosphorylates acidic proteins such as casein. This kinase is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. Casein kinase II (CSNK2A1) is a constitutively active, ubiquitously expressed serine / threonine protein kinase that is thought to have a regulatory function in cell proliferation, cell differentiation and apoptosis. CSNK2A1 functions as a tetrameric complex consisting of two regulatory beta-subunits and two catalytic units (alpha and alpha') in a homomeric or heteromeric conformation. Whilst the alpha- and alpha'-subunits are catalytically identical, proteins that regulate CSNK2A1, such as cdc2 and Hsp90, preferentially bind to the alpha and not the alpha'-subunit. CSNK2A1 can phosphorylate a number of key intracellular signaling proteins implicated in tumor suppression (p53 and PTEN) and tumorigenesis (myc, jun, NF-kappaB). CSNK2A1 is also thought to influence Wnt signaling via beta-catenin phosphorylation and the PI 3-K signaling pathway via the phosphorylation of Akt. |
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CK2 alpha/CSNK2A1 Protein, Human, Recombinant (GST) CK2 α/CSNK2A1 casein kinase 2, α 1 polypeptide casein kinase 2, a 1 polypeptide CKII CSNK2A3 CK2A1 casein kinase 2, α1 polypeptide casein kinase 2, alpha 1 polypeptide recombinant recombinant-proteins proteins protein