CK2 alpha/CSNK2A1 Protein, Mouse, Recombinant

CK2 alpha/CSNK2A1 Protein, Mouse, Recombinant is expressed in Baculovirus insect cells. The predicted molecular weight is 45.3 kDa and the accession number is Q60737.

CK2 alpha/CSNK2A1 Protein, Mouse, Recombinant

パッケージサイズ	在庫状況	単価(税別)
50 μg	約5 days	￥ 114,500

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説明	CK2 alpha/CSNK2A1 Protein, Mouse, Recombinant is expressed in Baculovirus insect cells. The predicted molecular weight is 45.3 kDa and the accession number is Q60737.

Species	Mouse
Expression Host	Baculovirus Insect Cells
Tag	Tag Free
Accession Number	Q60737
別名	casein kinase 2, α1 polypeptide, casein kinase 2, alpha 1 polypeptide, CK2 α/CSNK2A1, Csnk2a1-rs4
Construction	The Mouse CAMK4 CSNK2A1 (Q60737) (Met1-Gln391) was expressed and purified with two additional amino acids (Gly & Pro) at the N-terminus.
Protein Purity	> 90 % as determined by SDS-PAGE
分子量	45.3 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Supplied as sterile 20 mM Tris, 500 mM NaCl, 10% glycerol, pH 7.4.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store the product under sterile conditions at -20℃ to -80℃. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.
Research Background	Casein kinase II subunit alpha, also known as CK II alpha, CSNK2A1 and CK2A1, is a member of the protein kinase superfamily, Ser / Thr protein kinase family and CK2 subfamily. Casein kinase II (CSNK2A1) is a serine / threonine protein kinase that phosphorylates acidic proteins such as casein. This kinase is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. Casein kinase II (CSNK2A1) is a constitutively active, ubiquitously expressed serine / threonine protein kinase that is thought to have a regulatory function in cell proliferation, cell differentiation and apoptosis. CSNK2A1 functions as a tetrameric complex consisting of two regulatory beta-subunits and two catalytic units (alpha and alpha') in a homomeric or heteromeric conformation. Whilst the alpha- and alpha'-subunits are catalytically identical, proteins that regulate CSNK2A1, such as cdc2 and Hsp90, preferentially bind to the alpha and not the alpha'-subunit. CSNK2A1 can phosphorylate a number of key intracellular signaling proteins implicated in tumor suppression (p53 and PTEN) and tumorigenesis (myc, jun, NF-kappaB). CSNK2A1 is also thought to influence Wnt signaling via beta-catenin phosphorylation and the PI 3-K signaling pathway via the phosphorylation of Akt.

参考文献

1. Schlpfer J,et al.(1997) A radiation hybrid framework map of bovine chromosome 13. Chromosome Res. 5(8): 511-9. 2. Wirkner U,et al.(1994) The human gene (CSNK2A1) coding for the casein kinase II subunit alpha is located on chromosome 20 and contains tandemly arranged Alu repeats. Genomics. 19(2): 257-65. 3. Wirkner U,et al.(1998) Genomic organization and promoter identification of the human protein kinase CK2 catalytic subunit alpha (CSNK2A1). Genomics. 48(1): 71-8.

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CK2 alpha/CSNK2A1 Protein, Mouse, Recombinant

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