Claudin-6 Protein, Human, Recombinant (His & FLAG)

Claudin-6 Protein, Human, Recombinant (His & FLAG) is expressed in HEK293 mammalian cells with His and Flag tag. The predicted molecular weight is 21.87 kDa and the accession number is P56747.

Claudin-6 Protein, Human, Recombinant (His & FLAG)

パッケージサイズ	在庫状況	単価(税別)
20 μg	約5 days	￥ 555,500
100 μg	約5 days	￥ 1,948,500

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説明	Claudin-6 Protein, Human, Recombinant (His & FLAG) is expressed in HEK293 mammalian cells with His and Flag tag. The predicted molecular weight is 21.87 kDa and the accession number is P56747.

Species	Human
Expression Host	HEK293 Cells
Tag	His, Flag
Accession Number	P56747
別名	claudin 6
Construction	The Human CLDN6 (P56747) (Met1-Thr184) was expressed, with a Flag tag followed by a polyhistidine tag at the C-terminus.Nanodisc is a versatile tool for studying membrane proteins. Using styrene-maleic acid (SMA) copolymer, membrane proteins can be extracted directly from prokaryotic and eukaryotic expression systems in the absence of detergents to preserve the protein structure and function better. Compared to membrane scaffold proteins (MSPs) nanodiscs, SMA nanodiscs also have the advantage of preserving proteins' nature by maintaining native lipids surrounded without introducing any heterologous proteins, which allows studies of protein structure and functions in a native-like environment.
Protein Purity	≥ 90 % as determined by SDS-PAGE
分子量	21.87 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Supplied as 0.2 μm filtered solution in 10 mM HEPES, 50 mM NaCl, pH7.5 with glycerol as protectant.
Stability & Storage	It is recommended to store the product under sterile conditions at -70℃ or lower. Samples are stable for up to 3 months at -80℃. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
Research Background	Claudin-6 (CLDN6) belongs to the claudins family and is a transmembrane protein found in tight junctions with two extracellular loops and intracellular N and C tails. CLDN6 modulates the ion- and charge-specific permeability of the paracellular pathway in most epithelial tissues. It regulates the paracellular flux of cations in the kidney through forming heterotypic strands with other claudins and has a role in maintaining the lung epithelial barrier function. The CLDN6 gene is adjacent to another family member, CLDN9, on chromosome 16. Certain CLDN6-positive cancers, encompassing ovarian, endometrial, and testicular malignancies, have been shown to have their proliferation and invasiveness suppressed upon CLDN6 silencing.

参考文献

1. Qu H, et al. CLDN6: From Traditional Barrier Function to Emerging Roles in Cancers. Int J Mol Sci. 2021. 2. Du H, et al. Claudin 6: Therapeutic prospects for tumours, and mechanisms of expression and regulation (Review). Mol Med Rep. 2021. 3. Brad Screnci, et. al. Atomic-level specificity of Claudin 6 monoclonal antibodies isolated for treating solid tumors [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2022. 4. Huang L, et al. Downregulation of CLDN6 inhibits cell proliferation, migration, and invasion via regulating EGFR/AKT/mTOR signalling pathway in hepatocellular carcinoma. Cell Biochem Funct. 2020.

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Claudin-6 Protein, Human, Recombinant (His & FLAG)

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