Claudin-9 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 22.44 kDa and the accession number is O95484.
説明 | Claudin-9 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 22.44 kDa and the accession number is O95484. |
Species | Human |
Expression Host | HEK293 Cells |
Tag | C-His-Avi |
Accession Number | O95484 |
Construction | The Human CLDN9 (O95484) (Met1-Thr184) was expressed, with a polyhistidine tag followed by an AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.Nanodisc is a versatile tool for studying membrane proteins. Using styrene-maleic acid (SMA) copolymer, membrane proteins can be extracted directly from prokaryotic and eukaryotic expression systems in the absence of detergents to preserve the protein structure and function better. Compared to membrane scaffold proteins (MSPs) nanodiscs, SMA nanodiscs also have the advantage of preserving proteins' nature by maintaining native lipids surrounded without introducing any heterologous proteins, which allows studies of protein structure and functions in a native-like environment. |
Protein Purity | >80% as determined by SDS-PAGE. |
分子量 | 22.44 kDa (predicted) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Supplied as 0.2 μm filtered solution in 10 mM HEPES, 50 mM NaCl, pH7.5 with glycerol as protectant. |
Stability & Storage |
It is recommended to store the product under sterile conditions at -70℃ or lower. Samples are stable for up to 12 months at -80℃. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping |
In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice. |
Research Background | Claudin-9 (CLDN9) belongs to the claudins family and is a transmembrane protein found in tight junctions with two extracellular loops and a cytoplasmic C tail. CLDN9 modulates the ion- and charge-specific permeability of the paracellular pathway in most epithelial tissues. It forms heterotypic interactions with other claudins to create cation-selective channels in the kidney and may contribute to the maintenance of alveolar barrier function in the lung. Deficiency is shown to be associated with autosomal recessive deafness, DFNB116. CLDN9 expression has been shown to be upregulated in certain types of cancer, such as endometrial cancer and hepatocellular carcinoma, and its knockdown has been found to reduce cell proliferation and migration in vitro. |
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