CLEC12A/MICL/CLL-1 Protein, Human, Recombinant (hFc & Avi), Biotinylated

CLEC12A has recently been identified as an antigen, expressed on leukemic stem cells and leukemic blasts. Given the fact that this expression profile seems stable throughout diagnosis, treatment and relapse on leukemic blasts and leukemic stem cells, CLEC12A can be considered a highly potent and reliable marker for the detection of measurable residual disease and therefore applicable for risk stratification and prognostication in AML. CLEC12A/MICL/CLL-1 Protein, Human, Recombinant (hFc & Avi), Biotinylated is expressed in HEK293 mammalian cells with N-hFc-Avi tag. The predicted molecular weight is 51.7 kDa and the accession number is Q5QGZ9-2.

CLEC12A/MICL/CLL-1 Protein, Human, Recombinant (hFc & Avi), Biotinylated

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 187,000
500 μg	約5 days	￥ 746,000
1 mg	約5 days	￥ 1,248,500

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説明

Species	Human
Expression Host	HEK293
Tag	N-hFc-Avi
Accession Number	Q5QGZ9-2
別名	HECL, DCAL-2, CD303, CLECSF7, DCAL2, CLECSF11, CLL1, CLL-1, CD371, MICL, PRO34150, CLEC12A, DLEC
Construction	Recombinant Biotinylated Human CLEC12A/MICL/CLL-1 Protein is expressed from HEK293 with hFc tag and Avi tag at the N-Terminus. It contains His65-Ala265.[Accession \|Q5QGZ9-2]
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
分子量	The protein has a predicted MW of 51.7 kDa. Due to glycosylation, the protein migrates to 67-70 kDa based on Tris-Bis PAGE result.

Endotoxin	Less than 1EU per ug by the LAL method.
Formulation	Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability & Storage	-20 to -80°C for 12 months as supplied from date of receipt. -20 to -80°C for 3-6 months in unopened state after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	CLEC12A has recently been identified as an antigen, expressed on leukemic stem cells and leukemic blasts. Given the fact that this expression profile seems stable throughout diagnosis, treatment and relapse on leukemic blasts and leukemic stem cells, CLEC12A can be considered a highly potent and reliable marker for the detection of measurable residual disease and therefore applicable for risk stratification and prognostication in AML.

参考文献

1. Bill M, et al. Revisiting CLEC12A as leukaemic stem cell marker in AML: highlighting the necessity of precision diagnostics in patients eligible for targeted therapy. Br J Haematol. 2019 Mar;184(5):769-78doi: 10.1111/bjh.1571Epub 2018 Dec 5. PMID: 30520015.

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CLEC12A/MICL/CLL-1 Protein, Human, Recombinant (hFc & Avi), Biotinylated

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