CRISPR-Cas9 Protein, Streptococcus pyogenes, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 163 kDa and the accession number is Q99ZW2.
| 生物学的情報 |
1 μg = 5 U1 U is defined as cutting 1 ug 1 KB dsDNA in 30 min at 37℃
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| 説明 |
CRISPR-Cas9 Protein, Streptococcus pyogenes, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 163 kDa and the accession number is Q99ZW2. |
| Species |
Streptococcus pyogenes
|
| Expression Host |
Baculovirus Insect Cells
|
| Tag |
C-His
|
| Accession Number |
Q99ZW2
|
| 別名 |
SpyCas9
|
| Construction |
The s. pyogenes serotype M1 cas9 (WP_010922251.1) (Met1-Asp1368) was expressed with a nuclear localization sequence (NLS) at the N-terminus, two NLSs and a polyhistidine tag at the C-terminus.
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| Protein Purity |
≥ 90 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
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| 分子量 |
163 kDa (predicted)
|
| Endotoxin |
< 1.0 EU/μg of the protein as determined by the LAL method.
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| Formulation |
Supplied from sterile 20 mM Tris, 300 mM NaCl, 50% glycerol, pH 8.0, 0.5 mM PMSF, 0.1 mM EDTA, 0.5 mM TCEP.
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| Reconstitution |
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
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| Stability & Storage |
It is recommended to store the product under sterile conditions at -20℃ to -80℃. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
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| Shipping |
In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
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