CSK Protein, Human, Recombinant (GST)

CSK Protein, Human, Recombinant (GST) is expressed in Baculovirus insect cells with GST tag. The predicted molecular weight is 77 kDa and the accession number is P41240.

CSK Protein, Human, Recombinant (GST)

パッケージサイズ	在庫状況	単価(税別)
50 μg	約5 days	￥ 114,500

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説明	CSK Protein, Human, Recombinant (GST) is expressed in Baculovirus insect cells with GST tag. The predicted molecular weight is 77 kDa and the accession number is P41240.

Species	Human
Expression Host	Baculovirus Insect Cells
Tag	GST
Accession Number	P41240
別名	c-src tyrosine kinase
Construction	The Human CSK (NP_004374.1) (Met 1-Leu 450) was fused with the GST tag at the N-terminus.
Protein Purity	> 92 % as determined by SDS-PAGE
分子量	77 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Supplied as sterile 20 mM Tris, 500 mM NaCl, 0.5 mM PMSF, pH 7.4.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store the product under sterile conditions at -20℃ to -80℃. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.
Research Background	The tyrosine kinase c-Src has been implicated as a modulator of cell proliferation, spreading, and migration. These functions are also regulated by Met. The structure of a large fragment of the c-Src kinase comprises the regulatory and kinase domains and the carboxy-terminal tall. c-Src kinase interactions among domains and is stabilized by binding of the phosphorylated tail to the SH2 domain. This molecule is locked in a conformation that simultaneously disrupts the kinase active site and sequesters the binding surfaces of the SH2 and SH3 domains. The structure shows how appropriate cellular signals, or transforming mutations in v-Src, could break these interactions to produce an open, active kinase. The protein-tyrosine kinase activity of c-Src kinase is inhibited by phosphorylation of tyr527, within the c-Src c-terminal tail. Genetic and biochemical data have suggested that this negative regulation requires an intact Src homology 2 (SH2) domain. Since SH2 domains recognize phosphotyrosine, it is possible that these two non-catalytic domains associate, and thereby repress c-Src kinase activity. Experiments have suggested that c-Src kinase plays a role in the biological behaviour of colonic carcinoma cells induced by migratory factors such as EGF, perhaps acting in conjunction with FAK to regulate focal adhesion turnover and tumour cell motility. Furthermore, although c-Src kinase has been implicated in colonic tumour progression, in the adenoma to carcinoma in vitro model c-Src is not the driving force for this progression but co-operates with other molecules in carcinoma development.References

参考文献

1. Brauninger A. et al.,1992, Gene. 110: 205-11. 2. Sondhi D. et al., 1999, Biochemistry. 38 (34): 11147-55. 3. Ogawa A. et al., 2002, J Biol Chem. 277 (17): 14351-4. 4. Cole PA. et al., 2003, Curr Opin Chem Biol. 7 (5): 580-5. 5. Baumeister U. et al., 2005,EMBO J. 24 (9): 1686-95.

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CSK Protein, Human, Recombinant (GST)

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