CTLA-4 Protein, Human, Recombinant (His)

CTLA-4 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 15 kDa and the accession number is P16410-1.

CTLA-4 Protein, Human, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
100 μg	在庫あり	￥ 38,000

バルクのお問い合わせ

生物学的情報

1. Immobilized Recombinant Human CD86/B7-2 Protein (Fc Tag) at 2 μg/mL (100 μL/well) can bind Recombinant Human CTLA-4 Protein (His Tag) , the EC50 is 2.6-8 ng/mL.
2. Measured by its ability to inhibit IL-2 secretion by stimulated Jurkat human acute T cell leukemia cells. The ED50 for this effect is 0.05-0.3 μg/mL when stimulated with 1 μg/mL Recombinant Human B7‑1/CD80.
3. Captured Ipilimumab on proA Chip can bind CTLA-4 with an affinity constant of 20.03 nM as determined in a SPR assay (Routinely tested).
4. Loaded Ipilimumab on ProA Biosensor, can bind CTLA-4 Protein, Human, Recombinant, His Tag with an affinity constant of 5.15 nM as determined in BLI assay (Routinely tested).

説明

CTLA-4 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 15 kDa and the accession number is P16410-1.

Species	Human
Expression Host	HEK293 Cells
Tag	His
Accession Number	P16410-1
別名	ALPS5, CD152, GRD4, cytotoxic T-lymphocyte-associated protein 4, GSE, IDDM12, CELIAC3, CTLA-4, CD
Construction	The Human CTLA4 (NP_005205.2) extracellular domain (Met 1-Phe 162) was fused with the a polyhistidine tag at the C-terminus.
Protein Purity	> 90 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
分子量	15 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Cytotoxic T-lymphocyte protein 4, also known as CTLA4 and CD152, is a single-pass type I membrane protein and a member of the immunoglobulin superfamily. It is the second member of the CD28 receptor family. The ligands or counterreceptors for these two proteins are the B7 family members, CD80 (B7-1) and CD86 (B7-2). CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may play an important role in their functions. CD152 or cytotoxic T lymphocyte antigen-4 (CTLA-4) is an essential receptor involved in the negative regulation of T cell activation. Because of its profound inhibitory role, CD152 has been considered a sound susceptible candidate in autoimmunity and a persuasive target for cancer immunotherapy. In particular, recent evidence suggests that CD152 is also important in the homeostasis and function of a population of suppressive cells, termed regulatory T cells (Treg).Cancer ImmunotherapyCo-inhibitory Immune Checkpoint TargetsCTLA4 / CD152 Immune Checkpoint ProteinsImmune CheckpointImmune Checkpoint Detection: AntibodiesImmune Checkpoint Detection: ELISA AntibodiesImmune Checkpoint Detection: IP AntibodiesImmune Checkpoint Detection: WB AntibodiesImmune Checkpoint ProteinsImmune Checkpoint TargetsImmunotherapyTargeted Therapy

参考文献

1. Slavik JM,et al.(1999) CD28/CTLA-4 and CD80/CD86 families: signaling and function. Immunol Res. 19(1): 1-24. 2. Holmberg D,et al.(2005) CTLA-4 (CD152) and its involvement in autoimmune disease. Autoimmunity. 38(3): 225-33. 3. Chin LT,et al.(2008) Immune intervention with monoclonal antibodies targeting CD152 (CTLA-4) for autoimmune and malignant diseases. Chang Gung Med J. 31(1): 1-15.

Calculator

Reconstitution Calculator

Recombinant Proteins Dilute Calculator

Specific Activity Calculator

bottom

CTLA-4 Protein, Human, Recombinant (His)

参考文献

Calculator

技術サポート

Keywords