CXADR/CAR Protein, Human, Recombinant (His)

CXADR/CAR Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 25.6 kDa and the accession number is P78310-1.

CXADR/CAR Protein, Human, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
100 μg	在庫あり	￥ 82,000
200 μg	約5 days	￥ 144,500
500 μg	約5 days	￥ 305,500

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生物学的情報	Testing in progress
説明	CXADR/CAR Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 25.6 kDa and the accession number is P78310-1.

Species	Human
Expression Host	HEK293 Cells
Tag	C-His
Accession Number	P78310-1
別名	coxsackie virus and adenovirus receptor, CAR, CXADR, CAR4/6, HCAR
Construction	The Human CXADR (NP_001329.1) extracellular domain (Met 1-Gly 237) was fused with a polyhistidine tag at the C-terminus.
Protein Purity	≥ 92 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
分子量	25.6 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	CXADR (coxsackie virus and adenovirus receptor), also known as CAR, is a type I transmembrane glycoprotein belonging to the CTX family of the Ig superfamily, and is essential for normal cardiac development in the mouse. Proposed as a homophilic cell adhesion molecule, CXADR is a component of the epithelial apical junction complex that is essential for the tight junction integrity, and probably involved in transepithelial migration of polymorphonuclear leukocytes (PMN). Mature mouse CXADR structrually comprises a 218 aa extracellular domain (ECD) with a V-type (D1) and a C2-type (D2) Ig-like domain, a 21 aa transmembrane segment and a 17 aa intracellular domain, among which,D1 is thought to be responsible for homodimer formation in trans within tight junctions. The ECD of mouse CXADR shares 97%, 9% sequence identity with the corresponding regions of rat, human CXADR.

参考文献

1. Tomko, R.P. et al., 1997, Proc. Natl. Acad. Sci. U.S.A. 94 (7): 3352–3356. 2. van Raaij , M.J. et al., 2001, Structure. 8 (11): 1147–1155. 3. Cohen, C.J. et al., 2001, J. Biol. Chem. 276 (27): 25392–25398. 4. Carson, S.D. et al., 2002, Rev. Med. Virol. 11 (4): 219–226. 5. Selinka, H.C. et al., 2004, Med. Microbiol. Immunol. 193 (2-3): 127–131. 6. Philipson, L. et al., 2004, Curr. Top. Microbiol. Immunol. 273:87-111. 7. Raschperger, E. et al., 2006, Exp. Cell Res. 312: 1566-1580.

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CXADR/CAR Protein, Human, Recombinant (His)

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