CXCL1 Protein, Human, Recombinant (His & NusA)

CXCL1 Protein, Human, Recombinant (His & NusA) is expressed in E. coli expression system with His and NusA tag. The predicted molecular weight is 65 kDa and the accession number is P09341-1.

CXCL1 Protein, Human, Recombinant (His & NusA)

パッケージサイズ	在庫状況	単価(税別)
50 μg	約5 days	￥ 70,500

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説明	CXCL1 Protein, Human, Recombinant (His & NusA) is expressed in E. coli expression system with His and NusA tag. The predicted molecular weight is 65 kDa and the accession number is P09341-1.

Species	Human
Expression Host	E. coli
Tag	His, NusA
Accession Number	P09341-1
別名	CA21H, CPS1, CYP21, GRO1, GROa, SCYB1, NAP-3, MGSA-a, P450c21B, CAH1, MGSA, chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, α), CYP21B, chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha), FSP
Construction	The mature form of Human CXCL1 (P09341-1) (Ala 35-Asn 107) was fused with the polyhistidine-tagged NusA tag at the N-terminus.
Protein Purity	> 95 % as determined by SDS-PAGE
分子量	65 kDa (predicted)

Endotoxin	Please contact us for more information.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	The Chemokine (C-X-C motif) Ligand 1, CXCL1, is a small cytokine belonging to the CXC chemokine family that was previously called GRO1 oncogene, GRO?, KC, Neutrophil-activating protein 3 (NAP-3) and melanoma growth stimulating activity, alpha (MSGA-a). CXCL1 already known to be important in osteoarthritis (OA), as a novel target gene of transcription factor AP-2? in chondrocytes and support the important role of AP-2? in cartilage. CXCL1 is a potent neutrophil chemoattractant with recognized roles in angiogenesis and inflammation. CXCL1 is a novel immediate PTH/PTHrP-responsive gene. CXCL1 may act as a chemoattractant for osteoclast precursors. CXCL1 may also have important pro-nociceptive effects via its direct actions on sensory neurons, and may induce long-term changes that involve protein synthesis. CXCL1 plays a critical nonredundant role in the development of experimental Lyme arthritis and carditis via CXCR2-mediated recruitment of neutrophils into the site of infection. CXCL1 functions through CXCR2 to transactivate the EGFR by proteolytic cleavage of HB-EGF, leading to activation of MAPK signalling and increased proliferation of epithelial ovarian cancer (EOC) cells. It might limit tumor growth by reinforcing senescence early in tumorigenesis. Thus, CXCL1 plays a role in spinal cord development by inhibiting the migration of oligodendrocyte precursors and is involved in the processes of angiogenesis, inflammation, wound healing, and tumorigenesis.

参考文献

1. Wang JG,et al.(2008) The chemokine CXCL1/growth related oncogene increases sodium currents and neuronal excitability in small diameter sensory neurons. Mol Pain. 4: 38. 2. Acosta JC,et al.(2009) A role for CXCR2 in senescence, but what about in cancer? Cancer Res. 69(6): 2167-70.Onan D,et al.(2009) The chemokine Cxcl1 is a novel target gene of parathyroid hormone (PTH)/PTH-related protein in committed osteoblasts. Endocrinology. 150(5): 2244-53. 3. Ritzman AM,et al.(2010) The chemokine receptor CXCR2 ligand KC (CXCL1) mediates neutrophil recruitment and is critical for development of experimental Lyme arthritis and carditis. Infect Immun. 78(11): 4593-600. 4. Bolitho C,et al.(2010) The chemokine CXCL1 induces proliferation in epithelial ovarian cancer cells by transactivation of the epidermal growth factor receptor. Endocr Relat Cancer. 17(4): 929-40. 5. Wenke AK,et al.(2011) The transcription factor AP-2? regulates CXCL1 during cartilage development and in osteoarthritis. Osteoarthritis Cartilage. 19(2): 206-12.

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CXCL1 Protein, Human, Recombinant (His & NusA)

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