Cystatin F/CST7 Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 15.2 kDa and the accession number is O76096.
説明 | Cystatin F/CST7 Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 15.2 kDa and the accession number is O76096. |
Species | Human |
Expression Host | HEK293 Cells |
Tag | Tag Free |
Accession Number | O76096 |
別名 | cystatin F (leukocystatin), CMAP |
Construction | The mature form of the Human Cystatin F (NP_003641.3) (Met 1-His 145) with five amino acids (DDDDK) at the C-terminus was expressed and purified. |
Protein Purity | > 95 % as determined by SDS-PAGE |
分子量 | 15.2 kDa (predicted) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing 50 mM Tris, 100 mM NaCl, pH 8.0.Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping |
In general, Lyophilized powders are shipping with blue ice. |
Research Background | The cystatin superfamily members are important natural cysteine protease inhibitors present in a wide variety of organisms and are divided into three classes. Cystatin F, also known as leukocystatin and CMAP (Cystatin-like Metastasis-Associated Protein), is a type 2 cystatin and its expression is limited to hematopoietic cells, with the highest expression levels being observed in monocytes, dendritic cells, and certain types of T-cells. Furthermore, cystatin F mRNA becomes up-regulated during dendritic cell maturation, and thus suggests a specific role of cystatin F in immune regulation. Cystatin F is produced as a dimer, an inactive cathepsin inhibitor which is activated by chemical reduction. In addition, Cystatin F and its homologues have been observed expressing in various human cancer cell lines established from malignant tumors, and thus indicates a new diagnosis and prevention approach of certain human carcinomas metastasis. |
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Cystatin F/CST7 Protein, Human, Recombinant cystatin F (leukocystatin) leukocystatin cystatin F CMAP recombinant recombinant-proteins proteins protein