DCLK1 Protein, Human, Recombinant (aa 1-705, His & GST)

DCLK1 Protein, Human, Recombinant (aa 1-705, His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 106 kDa and the accession number is O15075-1.

DCLK1 Protein, Human, Recombinant (aa 1-705, His & GST)

パッケージサイズ	在庫状況	単価(税別)
50 μg	約5 days	￥ 114,500

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説明	DCLK1 Protein, Human, Recombinant (aa 1-705, His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 106 kDa and the accession number is O15075-1.

Species	Human
Expression Host	Baculovirus Insect Cells
Tag	His, GST
Accession Number	O15075-1
別名	doublecortin-like kinase 1, CL1, DCDC3A, DCLK, CLICK1, DCAMKL1
Construction	The Human DCLK1 (O15075-1) (Met 1-Val 705) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Protein Purity	> 84 % as determined by SDS-PAGE
分子量	106 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Supplied as sterile 20 mM Tris, 500 mM NaCl, pH 7.4, 10% gly, 0.5 mM PMSF.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store the product under sterile conditions at -20℃ to -80℃. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.
Research Background	DCAMKL1, also known as DCLK1, is a member of the protein kinase superfamily and the doublecortin family. It contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. DCAMKL1 is involved in several different cellular processes, including neuronal migration, retrograde transport, neuronal apoptosis and neurogenesis. Its microtubule-polymerizing activity is independent of its protein kinase activity. DCAMKL1 may be involved in a calcium-signaling pathway controlling neuronal migration in the developing brain. It may also participate in functions of the mature nervous system.

参考文献

1. Sossey-Alaoui K,et al. (1999) DCAMKL1, a brain-specific transmembrane protein on 13q12.3 that is similar to doublecortin (DCX). Genomics. 56 (1): 121-6. 2. Matsumoto N,et al. (1999) Genomic structure, chromosomal mapping, and expression pattern of human DCAMKL1 (KIAA0369), a homologue of DCX (XLIS). Genomics. 56 (2): 179-83. 3. Lin PT,et al. (2001) DCAMKL1 encodes a protein kinase with homology to doublecortin that regulates microtubule polymerization. J Neurosci. 20 (24): 9152-61.

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DCLK1 Protein, Human, Recombinant (aa 1-705, His & GST)

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