DDR2 Protein, Human, Recombinant (aa 422-855, His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 77.1 kDa and the accession number is Q16832.
説明 | DDR2 Protein, Human, Recombinant (aa 422-855, His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 77.1 kDa and the accession number is Q16832. |
Species | Human |
Expression Host | Baculovirus Insect Cells |
Tag | His, GST |
Accession Number | Q16832 |
別名 | discoidin domain receptor tyrosine kinase 2, NTRKR3, CD167, TYRO10, TKT, MIG20a |
Construction | The Human DDR2 (Q16832) (Arg422-Glu855) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus. |
Protein Purity | > 90 % as determined by SDS-PAGE |
分子量 | 77.1 kDa (predicted) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Supplied as sterile 20 mM Tris, 500 mM NaCl, pH 7.4, 10% glycerol. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage |
It is recommended to store the product under sterile conditions at -20℃ to -80℃. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping |
Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice. |
Research Background | Discoidin domain receptor 2 (DDR2) or CD167b (cluster of differentiation 167b) is a kind of protein tyrosine kinases associated with cell proliferation and tumor metastasis, and collagen, identified as a ligand for DDR2, up-regulates matrix metallloproteinase 1 (MMP-1) and MMP-2 expression in cellular matrix. DDR2/CD167b was found to recognise the triple-helical region of collagen X as well as the NC1 domain. Binding to the collagenous region was dependent on the triple-helical conformation. DDR2/CD167b autophosphorylation was induced by the collagen X triple-helical region but not the NC1 domain, indicating that the triple-helical region of collagen X contains a specific DDR2 binding site that is capable of receptor activation. DDR2/CD167b is induced during stellate cell activation and implicate the phosphorylated receptor as a mediator of MMP-2 release and growth stimulation in response to type I collagen. Moreover, type I collagen-dependent upregulation of DDR2/CD167b expression establishes a positive feedback loop in activated stellate cells, leading to further proliferation and enhanced invasive activity.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy |
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Please read the User Guide of Recombinant Proteins for more specific information.
DDR2 Protein, Human, Recombinant (aa 422-855, His & GST) NTRKR 3 discoidin domain receptor tyrosine kinase 2 TYRO-10 TYRO 10 NTRKR3 CD167 CD 167 TYRO10 NTRKR-3 CD-167 TKT MIG20a recombinant recombinant-proteins proteins protein