Dectin-1 Protein, Human, Recombinant

Dectin-1 Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 15.5 kDa and the accession number is Q9BXN2-2.

Dectin-1 Protein, Human, Recombinant

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 161,000
1 mg	約5 days	￥ 1,046,500

バルクのお問い合わせ

説明	Dectin-1 Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 15.5 kDa and the accession number is Q9BXN2-2.

Species	Human
Expression Host	HEK293 Cells
Tag	Tag Free
Accession Number	Q9BXN2-2
別名	C-type lectin domain family 7, member A, CLECSF12, DECTIN1, CANDF4, BGR
Construction	extracellular domain of the Human Dectin1 (NP_072092.2) (Thr66-Met201)
Protein Purity	> 90 % as determined by SDS-PAGE
分子量	15.5 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Dectin-1 was recently identified as the most important receptor for beta-glucan. It is a type II transmembrane protein which binds beta-1,3 and beta-1,6 glucans, and is expressed on most cells of the innate immune system and has been implicated in phagocytosis as well as killing of fungi by macrophages, neutrophils and dendritic cells. Recognition of beta-glucan by dectin-1 triggers effective immune response, including phagocytosis and proinflammatory factor production, to eliminate infecting fungi, which especially benefits immunocompromised patients against opportunistic fungal infection. In addition, dectin-1 is involved in the adaptive immune response as well as autoimmune diseases and immune tolerance. Dectin-1 can recognize and respond to live fungal pathogens and is being increasingly appreciated as having a key role in the innate responses to these pathogens. In addition to its exogenous ligands, Dectin-1 can recognize an unidentified endogenous ligand on T cells and may act as a co-stimulatory molecule. Recent studies have highlighted the importance of Dectin-1 in anti-fungal immunity, in both mice and humans, and have suggested a possible involvement of this receptor in the control of mycobacterial infections.

参考文献

1. Herre J, et al. (2004) The role of Dectin-1 in antifungal immunity. Crit Rev Immunol. 24(3): 193-203. 2. Brown GD. (2006) Dectin-1: a signalling non-TLR pattern-recognition receptor. 6(1): 33-43. 3. Sun L, et al. (2007) The biological role of dectin-1 in immune response. Int Rev Immunol. 26(5-6): 349-64. 4. Schorey JS, et al. (2008) The pattern recognition receptor Dectin-1: from fungi to mycobacteria. Curr Drug Targets. 9(2): 123-9. 5. Reid DM, et al. (2009) Pattern recognition: recent insights from Dectin-Curr Opin Immunol. 21(1): 30-7.

Calculator

Reconstitution Calculator

Recombinant Proteins Dilute Calculator

Specific Activity Calculator

bottom

Dectin-1 Protein, Human, Recombinant

参考文献

Calculator

技術サポート

Keywords