DKK1 N terminal Domain Protein, Human, Recombinant (hFc & Avi)

Dickkopf-1 (Dkk1), the founding and best-studied member of the Dkk family, functions as an antagonist of canonical Wnt/β-catenin. Dkk1 is considered to play a broad role in a variety of biological processes. DKK1 N terminal Domain Protein, Human, Recombinant (hFc & Avi) is expressed in HEK293 mammalian cells with C-hFc-Avi tag. The predicted molecular weight is 40.25 kDa and the accession number is O94907.

DKK1 N terminal Domain Protein, Human, Recombinant (hFc & Avi)

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 112,000
500 μg	約5 days	￥ 447,500
1 mg	約5 days	￥ 746,000

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説明

Species	Human
Expression Host	HEK293
Tag	C-hFc-Avi
Accession Number	O94907
別名	Dickkopf-1, Dkk1, hDkk-1, hDkk1, Dkk-1, SK, Dickkopf-related 1
Construction	Recombinant Human DKK1 N terminal Domain Protein is expressed from HEK293 with hFc tag and Avi tag at the C-Terminus. It contains Thr32-Asp142.[Accession \|O94907]
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
分子量	The protein has a predicted MW of 40.25 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.

Endotoxin	Less than 1EU per μg by the LAL method.
Formulation	Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability & Storage	-20 to -80°C for 12 months as supplied from date of receipt. -20 to -80°C for 3-6 months in unopened state after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	Dickkopf-1 (Dkk1), the founding and best-studied member of the Dkk family, functions as an antagonist of canonical Wnt/β-catenin. Dkk1 is considered to play a broad role in a variety of biological processes.

参考文献

1. Liang L, et al. Dkk1 exacerbates doxorubicin-induced cardiotoxicity by inhibiting the Wnt/β-catenin signaling pathway. J Cell Sci. 2019 May 16;132(10):jcs228478. doi: 10.1242/jcs.228478. PMID: 31028181.

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DKK1 N terminal Domain Protein, Human, Recombinant (hFc & Avi)

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