DKK1 Protein, Rhesus, Recombinant (N256Q, His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 27.2 kDa and the accession number is XP_001098844.1.
パッケージサイズ | 在庫状況 | 単価(税別) |
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20 μg | 在庫あり | ¥ 31,500 |
100 μg | 約5 days | ¥ 91,500 |
200 μg | 約5 days | ¥ 156,000 |
500 μg | 約5 days | ¥ 314,500 |
生物学的情報 |
Measured by its ability to inhibit Wnt3a-induced alkaline phosphatase production by C3H10T1/2 2A6 mouse embryonal fibroblast cells. The ED50 for this effect is typically 0.5-3 μg/ml in the presence of 10 ng/mL of Wnt3a.
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説明 | DKK1 Protein, Rhesus, Recombinant (N256Q, His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 27.2 kDa and the accession number is XP_001098844.1. |
Species | Rhesus |
Expression Host | HEK293 |
Tag | His |
Accession Number | XP_001098844.1 |
別名 | dickkopf WNT signaling pathway inhibitor 1 |
Construction | A DNA sequence encoding the rhesus DKK1 (XP_001098844.1) precursor (Met 1-His 266) (256 Asn / Gln) was expressed, fused with a C-terminal polyhistidine tag. |
Protein Purity |
> 95 % as determined by SDS-PAGE
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分子量 | 27.2 kDa (predicted) |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA. |
Reconstitution | A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information. |
Stability & Storage |
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Shipping |
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |
Research Background | Dickkopf (DKK) family proteins, consisting of DKK-1, DKK-2, DKK-3 and DKK-4, function as secreted Wnt antagonists by inhibiting Wnt coreceptors LRP5/6. DKK-1, DKK-2, and DKK-4 also bind cell surface Kremen-1 or Kremen-2 and promote the internalization of LRP5/6. Dickkopf related protein 1 (DKK-1) was initially identified as an inducer of head formation in Xenopus embryos. DKK-1 protein modulates Wnt signaling pathway during embryonic development. Increased levels of DKK-1 are found in the majority of lung cancers, esophageal squamous cell carcinomas, and hormone-resistant breast cancers, while DKK-1 expression is decreased in malignant melanoma and colorectal cancers. |
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Please read the User Guide of Recombinant Proteins for more specific information.
DKK1 Protein, Rhesus, Recombinant (N256Q, His) dickkopf WNT signaling pathway inhibitor 1 recombinant recombinant-proteins proteins protein