DLL1 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 82.82 kDa and the accession number is A0A384P5C6.
パッケージサイズ | 在庫状況 | 単価(税別) |
---|---|---|
50 μg | 在庫あり | ¥ 52,500 |
100 μg | 約5 days | ¥ 92,000 |
200 μg | 約5 days | ¥ 161,000 |
500 μg | 約5 days | ¥ 335,000 |
生物学的情報 | Testing in progress |
説明 | DLL1 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 82.82 kDa and the accession number is A0A384P5C6. |
Species | Human |
Expression Host | HEK293 Cells |
Tag | C-hFc |
Accession Number | A0A384P5C6 |
別名 | DELTA1, DL1, delta-like 1 (Drosophila), δ-like 1 (Drosophila), δ1, δ, Delta |
Construction | The Human DLL1 (NP_005609.3) (Met1-Gly540) was expressed with the Fc region of Human IgG1 at the C-terminus. |
Protein Purity |
≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
|
分子量 | 82.82 kDa (predicted) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing 10% Trehalose Dihydrate, 0.02% Tween 80, 20 mM Tris, 100 mM NaCl, PH8.0.Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping |
In general, Lyophilized powders are shipping with blue ice. |
Research Background | Delta-like protein 1(DLL1), also known as Delta1, a single-pass type I membrane protein which contains one DSL domain and eight EGF-like domains, acts as a ligand for Notch receptors, and positively regulates T-cell development. DLL1 is proteolytically processed in a similar manner to the Notch receptor, and it has been speculated to participate in bidirectional signaling. The proteolytic processing of DLL1 helps achieve an asymmetry in Notch signaling in initially equivalent myogenic cells and helps sustain the balance between differentiation and self-renewal. Interactions between DLL1 and Notch in trans activate the Notch pathway, whereas DLL1 binding to Notch in cis inhibits Notch signaling. DLL1 undergoes proteolytic processing in its extracellular domain by ADAM10. It had been demonstrated that DLL1 represents a substrate for several other members of the ADAM family. In co-transfected cells, DLL1 is constitutively cleaved by ADAM12, and the N-terminal fragment of DLL1 is released to medium. ADAM12-mediated cleavage of DLL1 is cell density-dependent, takes place in cis orientation, and does not require the presence of the cytoplasmic domain of ADAM12. Full-length DLL1, but not its N- or C-terminal proteolytic fragment, co-immunoprecipitates with ADAM12. By using a Notch reporter construct, we show that DLL1 processing by ADAM12 increases Notch signaling in a cell-autonomous manner. Furthermore, ADAM9 and ADAM17 have the ability to process DLL1. In contrast, ADAM15 does not cleave DLL1, although the two proteins still co-immunoprecipitate with each other. During fetal development, DLL1 is an essential Notch ligand in the vascular endothelium of large arteries to activate Notch1 and maintain arterial identity. DLL1-Notch signaling was required for VEGF receptor expression in fetal arteries. |
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DLL1 Protein, Human, Recombinant (hFc) Drosophila DELTA1 DELTA 1 DL1 DL-1 DL 1 delta-like 1 (Drosophila) delta-like 1 Δ-like 1 δ-like 1 (Drosophila) δ1 δ Delta Δ-1 Δ 1 DELTA-1 recombinant recombinant-proteins proteins protein