DNA-PKcs Protein, Dictyostelium discoideum, Recombinant (His & Myc) is expressed in E. coli.
| 説明 |
DNA-PKcs Protein, Dictyostelium discoideum, Recombinant (His & Myc) is expressed in E. coli. |
| Species |
Dictyostelium discoideum
|
| Expression Host |
E. coli
|
| Tag |
N-10xHis, C-Myc
|
| Accession Number |
Q54UC0
|
| 別名 |
DNA-PKcs, DNA-PK catalytic subunit, dnapkcs, DNA-dependent protein kinase catalytic subunit
|
| Amino Acid |
ELDYPFLIKGGEDLRLDQRIQQLFGIMNEILKRDTACNKRSLNVTTYQVVPMTSKVGIIEWLNDTKPLREILEEQLAHQLKTPRSNVSISKLESTKYHNDWINSFAKYLKPNSPVGPLYQQMFIHATRDDCAKKLEKQHSKVPENLLQNGIWSLSSSPESYLFIRNSFARSLASFSVCSYVIGIGDRHLENFLISQRDGRLIGIDFGHAFGTATQFLPIPELMPFRLTRQFTSFLRPLDSVGLLNHNMTYTLTALQNQKEILLTTMDVFVKEPLLDWSKLATRLVKEQGKHPKDTKNVWFPKQKIQIAKKKLELVNPAYITLEELSGSVHSGLPYEKALSE
|
| Construction |
3910-4250 aa
|
| Protein Purity |
> 85% as determined by SDS-PAGE.
|
| 分子量 |
46.1 kDa (predicted)
|
| Formulation |
Tris-based buffer, 50% glycerol
|
| Reconstitution |
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
|
| Stability & Storage |
Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
|
| Shipping |
In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
|
| Research Background |
Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Is recruited to DNA ends by the Ku70/Ku80 heterodimer and is involved in DNA non-homologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. This activity is only apparent when DNA damage is administered in G1 phase of the cell cycle. Required for efficient signaling of DNA double-stranded breaks via phosphorylation of H2AX during G1.
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