DOPA Decarboxylase/DDC Protein, Human, Recombinant (His)

DOPA Decarboxylase/DDC Protein, Human, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 55 kDa and the accession number is Q53Y41.

DOPA Decarboxylase/DDC Protein, Human, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
50 μg	約5 days	￥ 138,000

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説明	DOPA Decarboxylase/DDC Protein, Human, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 55 kDa and the accession number is Q53Y41.

Species	Human
Expression Host	Baculovirus Insect Cells
Tag	C-His
Accession Number	Q53Y41
別名	dopa decarboxylase (aromatic L-amino acid decarboxylase), AADC
Construction	The full length of Human DDC (NP_000781.1) (Met 1-Glu 480) was expressed with a polyhistidine tag at the C-terminus.
Protein Purity	≥ 90 % as determined by SDS-PAGE. ≥ 85 % as determined by SEC-HPLC.
分子量	55 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Supplied as sterile 50 mM Tris, 100 mM NaCl, pH 8, 10% glycerol.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store the product under sterile conditions at -20℃ to -80℃. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.
Research Background	Dopa Decarboxylase (DDC), also known as AADC and Aromatic-L-amino acid decarboxylase, is a 54 kDa member of the group II decarboxylase family of proteins.It is a vitamin B6-dependent homodimeric enzyme that catalyzes the decarboxylation of both L-3,4-dihydroxyphenylalanine (L-DOPA) and L-5-hydroxytryptophan to dopamine and serotonin, respectively, which are major mammalian neurotransmitters and hormones belonging to catecholamines and indoleamines. Since L-DOPA is regularly used to treat the symptoms of Parkinson's disease, the catalytic pathway is of particular research interest. Defects of DDC are associated with severe developmental delay, oculogyric crises (OGC), as well as autosomal recessive disorder AADC deficiency, an early onset inborn error in neurotransmitter metabolism which can lead to catecholamine and serotonin deficiency.

参考文献

1. Ichinose, H. et al.,1989,Biochem. Biophys. Res. Commun. 164: 1024-1030. 2. Lisa, J. S. et al., 1992, Genomics 13: 469-471. 3. Moore, P. S. et al.,1996, Biochem. J. 315:249-256. 4. Bertoldi, M. et al., 2003, Biochim. Biophys. Acta. 1647:42-47. 5. Vassilacopoulou, D. et al., 2004, Neurochem. Res. 29: 1817-1823. 6. Ma, J.Z., et al., 2005, Hum. Mol. Genet. 14: 1691-1698.

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DOPA Decarboxylase/DDC Protein, Human, Recombinant (His)

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