DOPA Decarboxylase/DDC Protein, Mouse, Recombinant (His)

DOPA Decarboxylase/DDC Protein, Mouse, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 55.2 kDa and the accession number is O88533.

DOPA Decarboxylase/DDC Protein, Mouse, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 161,000

バルクのお問い合わせ

説明	DOPA Decarboxylase/DDC Protein, Mouse, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 55.2 kDa and the accession number is O88533.

Species	Mouse
Expression Host	Baculovirus Insect Cells
Tag	C-His
Accession Number	O88533
別名	Aadc, dopa decarboxylase (aromatic L-amino acid decarboxylase)
Construction	The Mouse DDC (O88533) (Met 1-Glu 480) was expressed，with a C-terminal polyhistidine tag.
Protein Purity	> 90 % as determined by SDS-PAGE
分子量	55.2 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 50 mM Tris, 100 mM NaCl, pH 8.0, 20% gly, 3 mM DTT. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Dopa Decarboxylase (DDC), also known as AADC and Aromatic-L-amino acid decarboxylase, is a 54 kDa member of the group II decarboxylase family of proteins.It is a vitamin B6-dependent homodimeric enzyme that catalyzes the decarboxylation of both L-3,4-dihydroxyphenylalanine (L-DOPA) and L-5-hydroxytryptophan to dopamine and serotonin, respectively, which are major mammalian neurotransmitters and hormones belonging to catecholamines and indoleamines. Since L-DOPA is regularly used to treat the symptoms of Parkinson's disease, the catalytic pathway is of particular research interest. Defects of DDC are associated with severe developmental delay, oculogyric crises (OGC), as well as autosomal recessive disorder AADC deficiency, an early onset inborn error in neurotransmitter metabolism which can lead to catecholamine and serotonin deficiency.

参考文献

1. Ichinose, H. et al.,1989,Biochem. Biophys. Res. Commun. 164: 1024-1030. 2. Lisa, J. S. et al., 1992, Genomics 13: 469-471. 3. Moore, P. S. et al.,1996, Biochem. J. 315:249-256. 4. Bertoldi, M. et al., 2003, Biochim. Biophys. Acta. 1647:42-47. 5. Vassilacopoulou, D. et al., 2004, Neurochem. Res. 29: 1817-1823. 6. Ma, J.Z., et al., 2005, Hum. Mol. Genet. 14: 1691-1698.

Calculator

Reconstitution Calculator

Recombinant Proteins Dilute Calculator

Specific Activity Calculator

bottom

DOPA Decarboxylase/DDC Protein, Mouse, Recombinant (His)

参考文献

Calculator

技術サポート

Keywords