DPP4/CD26 Protein, Human, Recombinant (hFc)

DPP4/CD26 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 112 kDa and the accession number is P27487.

DPP4/CD26 Protein, Human, Recombinant (hFc)

パッケージサイズ	在庫状況	単価(税別)
50 μg	在庫あり	￥ 73,500
100 μg	約5 days	￥ 125,500
200 μg	約5 days	￥ 214,500
500 μg	約5 days	￥ 434,000

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生物学的情報

1. Measured by its ability to bind recombinant Cynomolgus CXCL12 in a functional ELISA. 2. Measured by its ability to bind recombinant Human SDF1b in a functional ELISA. 3. Loaded Fc-DPPIV on ProA Biosensor, can bind MERS-CoV Spike/RBD Protein fragment (RBD, aa 367-606, His Tag) with an affinity constant of 1.04 nM as determined by Octet RED System. 4. Loaded Fc-DPPIV on ProA Biosensor, can bind MERS-CoV Spike/S1 Protein (S1 Subunit, aa 1-725, His Tag) with an affinity constant of 0.02 nM as determined by Octet RED System. 5. Loaded Fc-DPPIV on ProA Biosensor, can bind MERS-CoV Spike/RBD Protein fragment (RBD, aa 367-606, His Tag) with an affinity constant of 1.04 nM as determined by Octet RED System. 6. Loaded Fc-DPPIV on ProA Biosensor, can bind MERS-CoV Spike/S1 Protein (S1 Subunit, aa 1-725, His Tag) with an affinity constant of 0.02 nM as determined by Octet RED System.

説明

DPP4/CD26 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 112 kDa and the accession number is P27487.

Species	Human
Expression Host	HEK293 Cells
Tag	N-hFc
Accession Number	P27487
別名	DPPIV, TP103, ADABP, CD26, ADCP2, dipeptidylpeptidase 4
Construction	The extracellular domain (Asn 29-Pro 766) of the mature form of Human DPPIV (NP_001926.2) was expressed with the fused Fc region of Human IgG1 at the N-terminus.
Protein Purity	≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
分子量	112 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Dipeptidyl peptidase-4 (DPP4) or adenosine deaminase complexing protein 2 (ADCP 2) or T-cell activation antigen CD26 is a serine exopeptidase belonging to the S9B protein family that cleaves X-proline dipeptides from the N-terminus of polypeptides, such as chemokines, neuropeptides, and peptide hormones. The enzyme is a type II transmembrane glycoprotein, expressed on the surface of many cell types. It is also present in serum and other body fluids in a truncated form (sCD26/DPPIV). The soluble CD26 (sCD26) as a tumour marker for the detection of colorectal cancer (CRC) and advanced adenomas. As both a regulatory enzyme and a signalling factor, DPP4 has been evaluated and described in many studies. DPP4 inhibition results in increased blood concentration of the incretin hormones glucagon-like peptide-1 (GLP-1) and gastric inhibitory polypeptide (GIP). This causes an increase in glucose-dependent stimulation, resulting in a lowering of blood glucose levels. Recent studies have shown that DPP4 inhibitors can induce a significant reduction in glycosylated haemoglobin (HbA(1c)) levels, either as monotherapy or as a combination with other antidiabetic agents. Research has also demonstrated that DPP4 inhibitors portray a very low risk of hypoglycaemia development, and are a new pharmacological class of drugs for treating Type 2 diabetes.

参考文献

1. Doupis J, et al. (2008) DPP4 inhibitors: a new approach in diabetes treatment. Adv Ther. 25(7): 627-43. 2. HAvre PA, et al. (2008) The role of CD26/dipeptidyl peptidase IV in cancer. Front Biosci. 13: 1634-45. 3. De Chiara L, et al. (2009) Soluble CD26 levels and its association to epidemiologic parameters in a sample population. Dis Markers. 7(6): 311-6. 4. Matteucci E, et al. (2009) Dipeptidyl peptidase-4 (CD26): knowing the function before inhibiting the enzyme. Curr Med Chem. 16(23): 2943-51.

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DPP4/CD26 Protein, Human, Recombinant (hFc)

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