DPP4/CD26 Protein, Human, Recombinant

DPP4/CD26 Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 85.4 kDa and the accession number is P27487.

DPP4/CD26 Protein, Human, Recombinant

パッケージサイズ	在庫状況	単価(税別)
50 μg	在庫あり	￥ 103,500
100 μg	約5 days	￥ 177,000
200 μg	約5 days	￥ 301,000
500 μg	約5 days	￥ 948,000

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生物学的情報

1. Measured by its ability to cleave the fluorogenic peptide substrate, Gly-Pro-7-amido-4-methylcoumarin (GP-AMC). The specific activity is > 2,500 pmoles/min/μg. 2. Using the Octet RED System, the affinity constant (Kd) of human DPP4 bound to Spike (HCoV-EMC/2012) was 20 nM. 3. Using the Octet RED System, the affinity constant (Kd) of human DPP4 bound to Spike (HCoV-EMC/2012) was 50 nM. 4. Using the Octet RED System, the affinity constant (Kd) of human DPP4 bound to Spike (HCoV-EMC/2012) (ECD, aa 1-1297) was 33 nM. 5. Using the Octet RED System, the affinity constant (Kd) of human DPP4 bound to Spike-His (aa 1-760) was 12 nM.

説明

DPP4/CD26 Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 85.4 kDa and the accession number is P27487.

Species	Human
Expression Host	HEK293
Tag	Tag Free
Accession Number	P27487
別名	TP103, CD26, ADCP2, DPPIV, ADABP, dipeptidylpeptidase 4
Construction	The native mature form of human DPPIV (NP_001926.2) extracellular domain (Asn 29-Pro 766) was expressed and purified.
Protein Purity	≥ 70 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
分子量	85.4 kDa (predicted)

Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method.
Formulation	Lyophilized from sterile 100mM NaCl, 50mM Tris, pH 7.5. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution	A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Stability & Storage	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	Dipeptidyl peptidase-4 (DPP4) or adenosine deaminase complexing protein 2 (ADCP 2) or T-cell activation antigen CD26 is a serine exopeptidase belonging to the S9B protein family that cleaves X-proline dipeptides from the N-terminus of polypeptides, such as chemokines, neuropeptides, and peptide hormones. The enzyme is a type II transmembrane glycoprotein, expressed on the surface of many cell types. It is also present in serum and other body fluids in a truncated form (sCD26/DPPIV). The soluble CD26 (sCD26) as a tumour marker for the detection of colorectal cancer (CRC) and advanced adenomas. As both a regulatory enzyme and a signalling factor, DPP4 has been evaluated and described in many studies. DPP4 inhibition results in increased blood concentration of the incretin hormones glucagon-like peptide-1 (GLP-1) and gastric inhibitory polypeptide (GIP). This causes an increase in glucose-dependent stimulation, resulting in a lowering of blood glucose levels. Recent studies have shown that DPP4 inhibitors can induce a significant reduction in glycosylated haemoglobin (HbA(1c)) levels, either as monotherapy or as a combination with other antidiabetic agents. Research has also demonstrated that DPP4 inhibitors portray a very low risk of hypoglycaemia development, and are a new pharmacological class of drugs for treating Type 2 diabetes.

参考文献

1. Doupis J, et al. (2008) DPP4 inhibitors: a new approach in diabetes treatment. Adv Ther. 25(7): 627-43. 2. HAvre PA, et al. (2008) The role of CD26/dipeptidyl peptidase IV in cancer. Front Biosci. 13: 1634-45. 3. De Chiara L, et al. (2009) Soluble CD26 levels and its association to epidemiologic parameters in a sample population. Dis Markers. 7(6): 311-6. 4. Matteucci E, et al. (2009) Dipeptidyl peptidase-4 (CD26): knowing the function before inhibiting the enzyme. Curr Med Chem. 16(23): 2943-51.

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DPP4/CD26 Protein, Human, Recombinant

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