DPYS Protein, Human, Recombinant

DPYS Protein, Human, Recombinant is expressed in Baculovirus insect cells. The predicted molecular weight is 56.8 kDa and the accession number is Q14117.

DPYS Protein, Human, Recombinant

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 138,000

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説明	DPYS Protein, Human, Recombinant is expressed in Baculovirus insect cells. The predicted molecular weight is 56.8 kDa and the accession number is Q14117.

Species	Human
Expression Host	Baculovirus Insect Cells
Tag	Tag Free
Accession Number	Q14117
別名	dihydropyrimidinase, DHPase, DHP
Construction	The Human DPYS (NP_001376.1) (Met1-Pro519) was fused with two additional amino acids (Gly&Pro) at the N-terminus.
Protein Purity	> 95 % as determined by SDS-PAGE
分子量	56.8 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 500 mM NaCl, 3 mM DTT, 10% glycerol, pH 8.0.Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	DPYS, also known as dihydropyrimidinase, belongs to the DHOase family, hydantoinase/dihydropyrimidinase subfamily. DPYS catalyzes the second step of the reductive pyrimidine degradation, the reversible hydrolytic ring opening of dihydropyrimidines. It can catalyzes the ring opening of 5,6-dihydrouracil to N-carbamyl-alanine and of 5,6-dihydrothymine to N-carbamyl-amino isobutyrate. DPYS is expressed at a high level in liver and kidney as a major 2.5-kb transcript and a minor 3.8-kb transcript. Defects in the DPYS gene are linked to dihydropyrimidinuria.

参考文献

1. Thomas HR. et al., 2008, Genomics. 18 (1): 25-35. 2. Thomas HR. et al., 2008, Pharmacogenet Genomics. 17 (11): 973-87. 3. Van Kuilenburg AB. et al., 2007, Mol Genet Metab. 91 (2): 157-64.

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DPYS Protein, Human, Recombinant

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