DR3/TNFRSF25 Protein, Human, Recombinant (hFc), Biotinylated

DR3/TNFRSF25 Protein, Human, Recombinant (hFc), Biotinylated is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 45.9 kDa and the accession number is Q93038-10.

DR3/TNFRSF25 Protein, Human, Recombinant (hFc), Biotinylated

パッケージサイズ	在庫状況	単価(税別)
20 μg	約5 days	￥ 70,500
100 μg	約5 days	￥ 167,000

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説明	DR3/TNFRSF25 Protein, Human, Recombinant (hFc), Biotinylated is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 45.9 kDa and the accession number is Q93038-10.

Species	Human
Expression Host	HEK293 Cells
Tag	C-hFc
Accession Number	Q93038-10
別名	TRAMP, tumor necrosis factor receptor superfamily, member 25, TR3, DDR3, TNFRSF12, APO-3, WSL-LR, DR3, WSL-1, LARD
Construction	The Human TNFRSF25 (NP_683867.1) (Met1-Gln199) was expressed with the Fc region of Human IgG1 at the C-terminus. The purified protein was biotinylated in vitro.
Protein Purity	> 90 % as determined by SDS-PAGE.
分子量	45.9 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Tumor necrosis factor receptor superfamily, member 25 (TNFRSF25), also known as Death receptor 3 (DR3) or TNFRSF12 is a member of the TNF-receptor superfamily. This receptor is expressed preferentially in the tissues enriched in lymphocytes, and it may play a role in regulating lymphocyte homeostasis. TNFRSF25/DR3/TNFRSF12 has been shown to stimulate NF-kappa B activity and regulate cell apoptosis. The signal transduction of this receptor is mediated by various death domain containing adaptor proteins. Knockout studies in mice suggested the role of this gene in the removal of self-reactive T cells in the thymus. Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported, most of which are potentially secreted molecules. The alternative splicing of this TNFRSF25 encoding gene in B and T cells encounters a programmed change upon T-cell activation, which predominantly produces full-length, membrane bound isoforms, and is thought to be involved in controlling lymphocyte proliferation induced by T-cell activation.

参考文献

1. Slebioda TJ,et al.(2011) Triggering of TNFRSF25 promotes CD8? T-cell responses and anti-tumor immunity. Eur J Immunol. 41(9): 606-11. 2. Fang L,et al.(2008) Essential role of TNF receptor superfamily 25 (TNFRSF25) in the development of allergic lung inflammation. J Exp Med. 205(5): 037-48. 3. Borysenko CW,et al.(2005) Comparative modeling of TNFRSF25 (DR3) predicts receptor destabilization by a mutation linked to rheumatoid arthritis. Biochem Biophys Res Commun. 328(3): 94-9.

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DR3/TNFRSF25 Protein, Human, Recombinant (hFc), Biotinylated

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