DYRK3 Protein, Human, Recombinant (His & GST)

カタログ番号 TMPY-04380

別名: REDK, RED, DYRK5, dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 3, hYAK3-2

Dual specificity tyrosine-phosphorylation-regulated kinase 3, also known as Regulatory erythroid kinase, REDK and DYRK3, is a nucleus protein which belongs to theprotein kinase superfamily, CMGC Ser/Thr protein kinase family and MNB/DYRK subfamily. DYRKs are an emerging family of dual-specificity kinases that play key roles in cell proliferation, survival, and development. DYRK3 contains oneprotein kinase domain. Isoform 1 and isoform 2 of DYRK3 are highly expressed in testis and in hematopoietic tissue such as fetal liver, and bone marrow. Isoform 2 of DYRK3 is the predominant form in testis. Isoform 1 of DYRK3 is the predominant form in fetal liver and bone marrow. Isoform 1 and isoform 2 are present at low levels in heart, pancreas, lymph node, and thymus. DYRK3 is a negative regulator of EPO-dependent erythropoiesis. It may place an upper limit on red cell production during stress erythropoiesis. DYRK3 inhibits cell death due to cytokine withdrawal in hematopoietic progenitor cells. It may also act by regulating CREB/CRE signaling. DYRK3 proved to effectively inhibit NFAT (nuclear factor of activated T cells) transcriptional response pathways and to co-immunoprecipitate with NFATc3. DYRK3 attenuates (and possibly apportions) red cell production selectively during anemia.

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DYRK3 Protein, Human, Recombinant (His & GST)

パッケージサイズ	在庫状況	単価(税別)
50 μg	約5 days	￥ 114,500

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生物学的特性に関する説明

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Product Properties

参考文献

説明

Species	Human
Expression Host	Baculovirus Insect Cells
Tag	His, GST
Accession Number	O43781-1
別名	REDK, RED, DYRK5, dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 3, hYAK3-2
Construction	The Human DYRK3 isoform 1 (O43781-1) (Met 1-Ser 588) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Protein Purity	> 85 % as determined by SDS-PAGE
分子量	93.5 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Supplied as sterile 20 mM Tris, 500 mM NaCl, 0.5 mM PMSF, 10% gly, pH 8.0.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store the product under sterile conditions at -20℃ to -80℃. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.
Research Background	Dual specificity tyrosine-phosphorylation-regulated kinase 3, also known as Regulatory erythroid kinase, REDK and DYRK3, is a nucleus protein which belongs to theprotein kinase superfamily, CMGC Ser/Thr protein kinase family and MNB/DYRK subfamily. DYRKs are an emerging family of dual-specificity kinases that play key roles in cell proliferation, survival, and development. DYRK3 contains oneprotein kinase domain. Isoform 1 and isoform 2 of DYRK3 are highly expressed in testis and in hematopoietic tissue such as fetal liver, and bone marrow. Isoform 2 of DYRK3 is the predominant form in testis. Isoform 1 of DYRK3 is the predominant form in fetal liver and bone marrow. Isoform 1 and isoform 2 are present at low levels in heart, pancreas, lymph node, and thymus. DYRK3 is a negative regulator of EPO-dependent erythropoiesis. It may place an upper limit on red cell production during stress erythropoiesis. DYRK3 inhibits cell death due to cytokine withdrawal in hematopoietic progenitor cells. It may also act by regulating CREB/CRE signaling. DYRK3 proved to effectively inhibit NFAT (nuclear factor of activated T cells) transcriptional response pathways and to co-immunoprecipitate with NFATc3. DYRK3 attenuates (and possibly apportions) red cell production selectively during anemia.

参考文献

1. Becker W., et al., 1998, J. Biol. Chem. 273: 25893-25902. 2. Himpel,S. et al., 2000,J Biol Chem 275 (4):2431-8. 3. Li,K. et al., 2002, J Biol Chem. 277 (49):47052-60. 4. Bogacheva,O. et al., 2008, J Biol Chem. 283 (52):36665-75. 5. Guo,X. et al., 2010, J Biol Chem.285 (17):13223-32.

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Keywords

DYRK3 Protein, Human, Recombinant (His & GST) REDK RED DYRK 5 DYRK5 dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 3 hYAK3-2 DYRK-5 recombinant recombinant-proteins proteins protein