E8L Protein, Monkeypox virus, Recombinant (His)

E8L is an important protein that mediates the invasion of monkeypox virus into host cells. In the process of invading and completing replication, E8L acts as a surface-binding protein of mature virion and can bind to chondroitin sulfate on the cell surface, so that the virus can attach to target cells.

E8L Protein, Monkeypox virus, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 96,000
500 μg	約5 days	￥ 383,500
1 mg	約5 days	￥ 643,000

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説明

Species	Monkeypox virus
Expression Host	E. coli
Tag	N-His
Accession Number	QJQ40248.1
別名	Cell surface-binding protein, Carbonic anhydrase homolog
Construction	Met1-Thr275
Protein Purity	> 95% as determined by Tris-Bis PAGE
分子量	32.92 kDa (predicted). The protein migrates to 50-60 kDa based on Tris-Bis PAGE result.

Endotoxin	< 1 EU/μg by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, 200 mM L-arginine (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	E8L is an important protein that mediates the invasion of monkeypox virus into host cells. In the process of invading and completing replication, E8L acts as a surface-binding protein of mature virion and can bind to chondroitin sulfate on the cell surface, so that the virus can attach to target cells.

参考文献

1. Shchelkunov SN, et al. Analysis of the monkeypox virus genome. Virology. 2002 Jun 5;297(2):172-94. doi: 10.1006/viro.2002.1446. PMID: 12083817. 2. Weaver JR, Isaacs SN. Monkeypox virus and insights into its immunomodulatory proteins. Immunol Rev. 2008 Oct;225:96-113. doi: 10.1111/j.1600-065X.2008.00691.x. PMID: 18837778; PMCID: PMC2567051. 3. Alkhalil A, et al. Inhibition of monkeypox virus replication by RNA interference. Virol J. 2009 Nov 4;6:188. doi: 10.1186/1743-422X-6-188. PMID: 19889227; PMCID: PMC2777875.

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E8L Protein, Monkeypox virus, Recombinant (His)

参考文献

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