ECD Protein, Human, Recombinant (His & GST)

ECD, also known as ecdysoneless homolog, belongs to the SGT1 family. It is highly expressed in muscle and heart. ECD is a novel promoter of mammalian cell cycle progression. This function is related to its ability to remove the repressive effects of Rb-family tumor suppressors on E2F transcription factors. It is a novel tumor-promoting factor that is differentially expressed in pancreatic cancer and potentially regulates glucose metabolism within cancer cells. ECD may also be a transcriptional activator required for the expression of glycolytic genes.

ECD Protein, Human, Recombinant (His & GST)

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 138,000

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説明

Species	Human
Expression Host	Baculovirus Insect Cells
Tag	His, GST
Accession Number	O95905-1
別名	HSGT1, ECD, GCR2, ecdysoneless cell cycle regulator
Construction	The Human ECD (O95905-1) (Met1-Thr621) was expressed with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Protein Purity	> 90 % as determined by SDS-PAGE
分子量	100.6 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 500 mM NaCl, 10% glycerol, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	ECD, also known as ecdysoneless homolog, belongs to the SGT1 family. It is highly expressed in muscle and heart. ECD is a novel promoter of mammalian cell cycle progression. This function is related to its ability to remove the repressive effects of Rb-family tumor suppressors on E2F transcription factors. It is a novel tumor-promoting factor that is differentially expressed in pancreatic cancer and potentially regulates glucose metabolism within cancer cells. ECD may also be a transcriptional activator required for the expression of glycolytic genes.

参考文献

1. Badzek S. et al., 2011, Wien Klin Wochenschr. 123 (23-24): 726-31. 2. Zhao X. et al., 2012, Breast Cancer Res Treat. 134 (1): 171-80. 3. Dey P. et al., 2012, Clin Cancer Res. 18 (22): 6188-98.

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ECD Protein, Human, Recombinant (His & GST)

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