ECE2 Protein, Human, Recombinant (hFc)

ECE2 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 105 kDa and the accession number is P0DPD8.

ECE2 Protein, Human, Recombinant (hFc)

パッケージサイズ	在庫状況	単価(税別)
50 μg	約5 days	￥ 138,000

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説明	ECE2 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 105 kDa and the accession number is P0DPD8.

Species	Human
Expression Host	HEK293 Cells
Tag	hFc
Accession Number	P0DPD8
別名	endothelin converting enzyme 2, KIAA0604, UNQ403/PRO740
Construction	The ectodomain of Human endothelin converting enzyme 2 isoform A (NP_055508.3) (Gly 199-Trp 883) was fused with the Fc region of Human IgG1 at the N-terminus.
Protein Purity	> 95 % as determined by SDS-PAGE
分子量	105 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 100 mM Glycine, 10 mM NaCl, 50 mM Tris, pH 7.5. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Endothelin-converting enzyme 2, also known as ECE-2, is a metalloprotease that possesses many properties consistent with it being a neuropeptide-processing enzyme. Endothelin-converting enzymes (ECEs) are the key enzymes in the endothelin (ET) biosynthesis that catalyze the conversion of big ET, the biologically inactive precursor of mature ET. Two enzymes, termed ECE-1 and ECE-2, have been molecularly identified. ECE-2 is found primarily in neural tissues, with high levels of expression in midbrain, cerebellum, hypothalamus, frontal cortex and spinal cord and moderate levels in hippocampus and striatum. ECE-2 is strongly down-regulated in inferior parietal lobe from Alzheimer disease patients (at protein level). ECE-2 converts big endothelin-1 to endothelin-1. It is involved in the processing of various neuroendocrine peptides, including neurotensin, angiotensin I, substance P, proenkephalin-derived peptides, and prodynorphin-derived peptides. ECE-2 may limit beta-amyloid peptide accumulation in brain. It may also have methyltransferase activity. A comparison of residues around the cleavage site revealed that ECE-2 exhibits a unique cleavage site selectivity that is related to but distinct from that of ECE-1.

参考文献

1. Lorenzo M.-N.,et al.,(2001), Human endothelin converting enzyme-2 (ECE2): characterization of mRNA species and chromosomal localization. Biochim. Biophys. Acta 1522:46-52. 2. Muzny D.M.,et al., (2006), The DNA sequence, annotation and analysis of human chromosome 3.Nature 440:1194-1198. 3. Nagase T.,et al.,(1998), Prediction of the coding sequences of unidentified human genes. IX. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro.DNA Res. 5:31-39.

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ECE2 Protein, Human, Recombinant (hFc)

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