EFNB2A Protein, Danio rerio (zebrafish), Recombinant (hFc)

EFNB2A Protein, Danio rerio (zebrafish), Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 48.9 kDa and the accession number is O73874.

EFNB2A Protein, Danio rerio (zebrafish), Recombinant (hFc)

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 106,000

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説明	EFNB2A Protein, Danio rerio (zebrafish), Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 48.9 kDa and the accession number is O73874.

Species	Danio rerio (zebrafish)
Expression Host	HEK293 Cells
Tag	C-hFc
Accession Number	O73874
別名	EFNB2A, efnb2, id:ibd572
Construction	The zebrafish EFNB2A (O73874) (Met 1-Ala222) was expressed with the Fc region of Human IgG1 at the C-terminus.
Protein Purity	> 90 % as determined by SDS-PAGE
分子量	48.9 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, PH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Efnb2a, also known as ephrin B2a, belongs to the ephrin family. Members of this family are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Efnb2a binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Efnb2a, together with ephb4 may play a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration.

参考文献

1. Shaw KM. et al., 2006, Dev Dyn. 235 (7): 1753-60. 2. Kemp HA. et al., 2009, Dev Biol. 327 (2): 313-26.Patten SA. et al., 2012, PLoS One. 7 (2): e31650.

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EFNB2A Protein, Danio rerio (zebrafish), Recombinant (hFc)

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