EGFL7 Protein, Human, Recombinant (His)

EGFL7 Protein, Human, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 29.4 kDa and the accession number is A0A024R8F5.

EGFL7 Protein, Human, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 142,000
1 mg	約5 days	￥ 922,500

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説明	EGFL7 Protein, Human, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 29.4 kDa and the accession number is A0A024R8F5.

Species	Human
Expression Host	Baculovirus Insect Cells
Tag	His
Accession Number	A0A024R8F5
別名	VE-STATIN, NEU1, EGF-like-domain, multiple 7, ZNEU1
Construction	The mature form of Human EGFL7 (NP_057299.1) (Tyr 24-Ser 273) was expressed, with a polyhistidine tag at the N-terminus.
Protein Purity	> 85 % as determined by SDS-PAGE
分子量	29.4 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 100 mM Glycine, 10 mM NaCl, 10% glycerol, pH 7.0.Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Epidermal growth factor-like protein 7, also known as EGF-like protein 7, Multiple epidermal growth factor-like domains protein 7, Multiple EGF-like domains protein 7, Vascular endothelial statin, NOTCH4-like protein and EGFL7, is a secreted protein which contains twoEGF-like domains and oneEMI domain. EGFL7 was identified by a number of groups as a putative secreted factor produced by the vascular endothelial cells (ECs). EGFL7 is described as a novel endothelial cell-derived factor involved in the regulation of the spatial arrangement of cells during vascular tube assembly. With its impact on tubulogenesis and vessel shape EGFL7 joined the large family of molecules governing blood vessel formation. EGFL7 regulates midline angioblast migration in zebrafish embryos-a key step in vascular tubulogenesis. EGFL7 is tightly associated with the extracellular matrix (ECM), and it supports EC migration either as a single factor or in combination with other ECM molecules. EGFL7 provides a proper microenvironment for endothelial cell migration, thereby enabling accurate patterning. Our study indicates that the molecular composition of the ECM influences vascular morphogenesis. EGFL7 also regulates vascular tubulogenesis. It inhibits platelet-derived growth factor (PDGF)-BB-induced smooth muscle cell migration and promotes endothelial cells adhesion to the substrate.

参考文献

1. Fitch,M.J. et al., 2004, Dev Dyn. 230 (2):316-24. 2. Schmidt,M. et al., 2007, Novartis Found Symp. 283 :18-28. 3. Campagnolo,L.et al., 2008, Gene Expr Patterns 8 (6):389-96. 4. Picuric,S. et al., 2009, Protein Expr Purif. 68 (1):1-6. 5. Nikolic,I. et al., 2010, J Angiogenes Res. 2 (1): 9.

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EGFL7 Protein, Human, Recombinant (His)

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