Ectonucleotide pyrophosphatase/phosphodiesterase (ENPP)-1 is a membrane-bound protein that catalyzes the hydrolysis of extracellular nucleoside triphosphates to monophosphate and extracellular inorganic pyrophosphate (ePPi). Mechanical stimulation regulates ENPP-1 expression. ENPP1 Protein, Human, Recombinant (C-His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 96.5 kDa and the accession number is P22413.
パッケージサイズ | 在庫状況 | 単価(税別) |
---|---|---|
100 μg | 約5 days | ¥ 117,500 |
500 μg | 約5 days | ¥ 468,500 |
1 mg | 約5 days | ¥ 782,500 |
説明 | Ectonucleotide pyrophosphatase/phosphodiesterase (ENPP)-1 is a membrane-bound protein that catalyzes the hydrolysis of extracellular nucleoside triphosphates to monophosphate and extracellular inorganic pyrophosphate (ePPi). Mechanical stimulation regulates ENPP-1 expression. ENPP1 Protein, Human, Recombinant (C-His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 96.5 kDa and the accession number is P22413. |
Species | Human |
Expression Host | HEK293 Cells |
Tag | C-His |
Accession Number | P22413 |
別名 | M6S1, E-NPP 1, PCA1, PDNP1, ENPP1, NPPase, NPPS, M6S1NPP1, PCA1ARHR2, PC1, NPP1, Ly-41 Antigen |
Construction | Lys98-Asp925 |
Protein Purity | > 95% as determined by Tris-Bis PAGE |
分子量 | 96.5 kDa (predicted). Due to glycosylation, the protein migrates to 110-115 kDa based on Tris-Bis PAGE result. |
Endotoxin | < 1 EU/μg by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping |
In general, Lyophilized powders are shipping with blue ice. |
Research Background | Ectonucleotide pyrophosphatase/phosphodiesterase (ENPP)-1 is a membrane-bound protein that catalyzes the hydrolysis of extracellular nucleoside triphosphates to monophosphate and extracellular inorganic pyrophosphate (ePPi). Mechanical stimulation regulates ENPP-1 expression. |
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ENPP1 Protein, Human, Recombinant (C-His) PDNP-1 ENPP-1 PCA-1 NPP 1 PDNP 1 M6S1 PC-1 E-NPP 1 PCA1 PDNP1 NPP-1 ENPP1 PCA 1 ENPP 1 NPPase PC 1 NPPS M6S1NPP1 PCA1ARHR2 PC1 NPP1 Ly-41 Antigen recombinant recombinant-proteins proteins protein