ENSA Protein, Human, Recombinant (His), Biotinylated

ENSA Protein, Human, Recombinant (His), Biotinylated is expressed in E. coli expression system with His tag. The predicted molecular weight is 15.2 kDa and the accession number is O43768-1.

ENSA Protein, Human, Recombinant (His), Biotinylated

パッケージサイズ	在庫状況	単価(税別)
20 μg	約5 days	￥ 70,500
100 μg	約5 days	￥ 167,000

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説明	ENSA Protein, Human, Recombinant (His), Biotinylated is expressed in E. coli expression system with His tag. The predicted molecular weight is 15.2 kDa and the accession number is O43768-1.

Species	Human
Expression Host	E. coli
Tag	N-His
Accession Number	O43768-1
別名	ARPP-19e, endosulfine alpha, endosulfine α
Construction	The mature form of Human ENSA (O43768-1) (Met1-Glu121) was expressed with a polyhistide tag at the N-terminus. The purified protein was biotinylated in vitro.
Protein Purity	> 90 % as determined by SDS-PAGE
分子量	15.2 kDa (predicted)

Endotoxin	Please contact us for more information.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Endosulfine alpha, also known as ENSA, belongs to the endosulfine family. It is a highly conserved cAMP-regulated phosphoprotein (ARPP) family. Endosulfine alpha is widely expressed with high levels in skeletal muscle and brain and lower levels in the pancreas. As a protein phosphatase inhibitor, ENSA specifically inhibits protein phosphatase 2A (PP2A) during mitosis. When phosphorylated at Ser-67 during mitosis, specifically interacts with PPP2R2D (PR55-delta) and inhibits its activity, leading to inactivation of PP2A, an essential condition to keep cyclin-B1-CDK1 activity high during M phase By similarity. Endosulfine alpha also acts as a stimulator of insulin secretion by interacting with sulfonylurea receptor (ABCC8), thereby preventing sulfonylurea from binding to its receptor and reducing K(ATP) channel currents.

参考文献

1. Ye M. et al., 2001, Genome Res. 10 (10): 1546-60. 2. Apiou F. et al., 1999, Diabetes 48 (9): 1873-6. 3. Lennon G. et al., 1997, Genome Res. 6 (9): 791-806.

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ENSA Protein, Human, Recombinant (His), Biotinylated

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