EpCAM/TROP1 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 30.7 kDa and the accession number is P16422.
パッケージサイズ | 在庫状況 | 単価(税別) |
---|---|---|
20 μg | 在庫あり | ¥ 68,000 |
100 μg | 約5 days | ¥ 158,500 |
200 μg | 約5 days | ¥ 271,000 |
500 μg | 約5 days | ¥ 546,500 |
生物学的情報 | Measured by its ability to bind Human E-Cadherin Protein, Recombinant (ECD, hFc Tag) in functional ELISA. |
説明 | EpCAM/TROP1 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 30.7 kDa and the accession number is P16422. |
Species | Human |
Expression Host | HEK293 Cells |
Tag | His, Avi |
Accession Number | P16422 |
別名 | HNPCC8, EGP314, KSA, KS1/4, TROP1, ESA, DIAR5, TROP-1, MIC18, EGP40, MK-1, M4S1, EGP-2, TACSTD1, epithelial cell adhesion molecule |
Construction | The Human EPCAM (NP_002345.2) (Met1-Lys265) was expressed with a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. |
Protein Purity |
> 90 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC.
|
分子量 | 30.7 kDa (predicted) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping |
In general, Lyophilized powders are shipping with blue ice. |
Research Background | Epithelial Cell Adhesion Molecule (EpCAM), also known as GA733-2 antigen, is a type I transmembrane glycoprotein composed of an extracellular domain with two EGF-Like repeats and a cystenin-rich region, a transmembrane domain and a cytoplasmic domain. It modulates cell adhesion and proliferation. Its overexpression has been detected in many epithelial tumours and has been associated with high stage, high grade and a worse survival in some tumour types. EpCAM has been shown to function as a calcium-independent homophilic cell adhesion molecule that does not exhibit any obvious relationship to the four known cell adhesion molecule superfamilies. However, recent insights have revealed that EpCAM participates in not only cell adhesion, but also in proliferation, migration and differentiation of cells. In addition, recent study revealed that EpCAM is the Wnt-beta-catenin signaling target gene and may be used to facilitate prognosis. It has oncogenic potential and is activated by release of its intracellular domain, which can signal into the cell nucleus by engagement of elements of the wnt pathway.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy |
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Please read the User Guide of Recombinant Proteins for more specific information.
EpCAM/TROP1 Protein, Human, Recombinant (His & Avi), Biotinylated HNPCC8 EGP2 EGP-314 EGP314 KSA HNPCC-8 KS1/4 MK 1 HNPCC 8 TROP1 MIC-18 ESA DIAR5 TROP-1 EGP-40 MIC18 DIAR 5 MK1 EGP40 EGP 2 MK-1 TROP 1 EGP 40 DIAR-5 EGP 314 MIC 18 M4S1 EGP-2 TACSTD1 epithelial cell adhesion molecule TACSTD 1 TACSTD-1 recombinant recombinant-proteins proteins protein