EphB3 Protein, Human, Recombinant (His)

Although EphB3 expression is down-regulated in colorectal cancer (CRC) cells compared with normal intestinal epithelial cells.EphB3 is down-regulated in CRC compared to normal mucosa. Hypermethylation of CpG island is contributed to downregulation of EphB3 in CRC. EphB3 expression in tumor cells may be a useful prognostic indicator for patients with CRC.

EphB3 Protein, Human, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 96,000
500 μg	約5 days	￥ 383,500
1 mg	約5 days	￥ 643,000

バルクのお問い合わせ

説明

Species	Human
Expression Host	HEK293 Cells
Tag	C-His
Accession Number	P54753
別名	EC 2.7.10.1, EPHB3, ETK2, EK2, HEK2, AW456895, Sek4, MDK5, Cek10, TYRO6, EC 2.7.10
Construction	Gly34-Leu559
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
分子量	58.6 kDa (predicted). Due to glycosylation, the protein migrates to 65-68 kDa based on Tris-Bis PAGE result.

Endotoxin	< 1 EU/μg by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 150 mM NaCl (pH 7.5). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Although EphB3 expression is down-regulated in colorectal cancer (CRC) cells compared with normal intestinal epithelial cells.EphB3 is down-regulated in CRC compared to normal mucosa. Hypermethylation of CpG island is contributed to downregulation of EphB3 in CRC. EphB3 expression in tumor cells may be a useful prognostic indicator for patients with CRC.

参考文献

1. Xuan Z, et al. Receptor Tyrosine Kinase EphB3: a Prognostic Indicator in Colorectal Carcinoma. Pathol Oncol Res. 2020 Jan;26(1):541-549. doi: 10.1007/s12253-018-0562-x. Epub 2018 Dec 7. PMID: 30535864.

Calculator

Reconstitution Calculator

Recombinant Proteins Dilute Calculator

Specific Activity Calculator

bottom

EphB3 Protein, Human, Recombinant (His)

参考文献

Calculator

技術サポート

Keywords