EphB4 Protein, Mouse, Recombinant (hFc)

EphB4 Protein, Mouse, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 84.7 kDa and the accession number is P54761-1.

EphB4 Protein, Mouse, Recombinant (hFc)

パッケージサイズ	在庫状況	単価(税別)
200 μg	在庫あり	￥ 73,500

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生物学的情報	Measured by its binding ability in a functional ELISA. Immobilized Mouse Ephrin B2 His at 2 μg/ml (100 μl/well) can bind Mouse EphB4 hFc , the EC50 of Mouse EphB4 hFc is 2.0-10.0 ng/mL.
説明	EphB4 Protein, Mouse, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 84.7 kDa and the accession number is P54761-1.

Species	Mouse
Expression Host	HEK293 Cells
Tag	hFc
Accession Number	P54761-1
別名	MDK2, Htk, Myk1, AI042935, Tyro11, EPH receptor B4
Construction	The Mouse EPHB4 (P54761-1) extracellular domain (Met 1-Ala 539) was fused with the Fc region of Human IgG1 at the C-terminus.
Protein Purity	> 97 % as determined by SDS-PAGE
分子量	84.7 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Ephrin type-B receptor 4 is a protein that in humans is encoded by the EPHB4 gene. It is a single-pass type I membrane protein belonging to the ephrin receptor subfamily of protein kinase superfamily. Members of the ephrin and Eph family are local mediators of cell function through largely contact-dependent processes in development and in maturity. Furthermore, EphB4 protein and the corresponding ligand Ephrin-B2 contribute to tumor growth in various human tumors. EphB4 protein has tumor suppressor activities and that regulation of cell proliferation, extracellular matrix remodeling, and invasive potential are important mechanisms of tumor suppression. Therefore, Ephrin-B2/EphB4 may be recognized as a novel prognostic indicator for cancers.

参考文献

1. Davalos V, et al. (2006) EPHB4 and survival of colorectal cancer patients. Cancer Res. 66(18): 8943-8. 2. Zhao C, et al. (2006) Bidirectional ephrinB2-EphB4 signaling controls bone homeostasis. Cell Metab. 4(2): 111-21. 3. Kertesz N, et al. (2006) The soluble extracellular domain of EphB4 (sEphB4) antagonizes EphB4-EphrinB2 interaction, modulates angiogenesis, and inhibits tumor growth. Blood. 107(6): 2330-8. 4. Noren NK, et al. (2007) Paradoxes of the EphB4 receptor in cancer. Cancer Res. 67(9): 3994-7. 5. Taylor AC, et al. (2007) EphB4 expression along adult rat microvascular networks: EphB4 is more than a venous specific marker. Microcirculation. 14(3): 253-67.

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EphB4 Protein, Mouse, Recombinant (hFc)

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