EPO/Erythropoietin Protein, Mouse, Recombinant

EPO/Erythropoietin Protein, Mouse, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 18.6 kDa and the accession number is P07321.

EPO/Erythropoietin Protein, Mouse, Recombinant

パッケージサイズ	在庫状況	単価(税別)
20 μg	在庫あり	￥ 82,500
100 μg	約5 days	￥ 282,500
200 μg	約5 days	￥ 480,000

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生物学的情報	Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 2-10 ng/mL.
説明	EPO/Erythropoietin Protein, Mouse, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 18.6 kDa and the accession number is P07321.

Species	Mouse
Expression Host	HEK293 Cells
Tag	Tag Free
Accession Number	P07321
別名	erythropoietin
Construction	Mouse Epo (NP_031968.1) (Met1-Arg192)
Protein Purity	> 95 % as determined by SDS-PAGE.
分子量	18.6 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Erythropoietin is a member of the EPO / TPO family. It is a secreted, glycosylated cytokine composed of four alpha helical bundles. Erythropoietin can be found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. It also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. Erythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass. It is produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals. Genetic variation in erythropoietin is associated with susceptbility to microvascular complications of diabetes type 2. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. It has a longer circulating half-life in vivo. Erythropoietin is being much misused as a performance-enhancing drug in endurance athletes.

参考文献

1. Jelkmann W, et al. (2007) Erythropoietin after a century of research: younger than ever. Eur J Haematol. 78 (3):183-205. 2. Miyake T, et al. (1997) Purification of human erythropoietin. J Biol Chem. 252(15):5558-64. 3. HAroon ZA, et al. (2003) A novel role for erythropoietin during fibrin-induced wound-healing response. Am J Pathol. 163(3):993-1000. 4. Siren AL, et al. (2001) Erythropoietin prevents neuronal apoptosis after cerebral ischemia and metabolic stress. Proc Natl Acad Sci. 98(7):4044-9.

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EPO/Erythropoietin Protein, Mouse, Recombinant

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