Complement Factor D/CFD Protein, Human, Recombinant (His)

Complement Factor D/CFD Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 25.8 kDa and the accession number is P00746.

Complement Factor D/CFD Protein, Human, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
50 μg	約5 days	￥ 114,500
500 μg	約5 days	￥ 594,500

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説明	Complement Factor D/CFD Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 25.8 kDa and the accession number is P00746.

Species	Human
Expression Host	HEK293 Cells
Tag	His
Accession Number	P00746
別名	PFD, DF, complement factor D (adipsin), ADN, FACTOR D, ADIPSIN
Construction	The mature form of Human CFD (P00746) (Ile 26-Ala 253) was fused with a C-terminal polyhistidine tag and a signal peptide at the N-terminus.
Protein Purity	> 97 % as determined by SDS-PAGE
分子量	25.8 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Complement factor D, also known as Adipsin, C3 convertase activator, Properdin factor D and CFD is a secreted protein which belongs to thepeptidase S1 family. CFD / Adipsin contains onepeptidase S1 domain. Complement factor D ( CFD / Adipsin ) is a component of the alternative complement pathway best known for its role in humoral suppression of infectious agents. Complement factor D ( CFD / Adipsin ) has a high level of expression in fat, suggesting a role for adipose tissue in immune system biology. This protein is also a serine protease that is secreted by adipocytes into the bloodstream. Complement factor D ( CFD / Adipsin ) cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway. Complement factor D ( CFD / Adipsin ) is a serine protease that stimulates glucose transport for triglyceride accumulation in fats cells and inhibits lipolysis. Defects in CFD / Adipsin are the cause of complement factor D deficiency (CFD deficiency) which predisposes to invasive meningococcal disease.

参考文献

1. Volanakis JE, et al.,1996, Protein Sci. 5 (4): 553-64. 2. Searfoss,G.H et al., 2003, J Biol Chem. 278 (46):46107-16. 3. Ukkola,O. et al., 2003, Eur J Clin Nutr. 57 (9):1073-8. 4. Ronti T, et al., 2006, Clin. Endocrinol. (Oxf) 64 (4): 355-65.

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Complement Factor D/CFD Protein, Human, Recombinant (His)

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