FCGR4 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 22.4 kDa and the accession number is A0A0B4J1G0.
| 生物学的情報 |
1.Measured by its binding ability in a functional ELISA. Immobilized mouse FCGR4 at 10 μg/ml (100 μl/well) can bind recombinant human IgG1 (Fc) . The EC50 of human IgG1 (Fc) is 0.11-0.25 μg/ml.
2.Loaded Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a on ProA Biosensor, can bind Recombinant Mouse FCGR4 Protein, His Tag with an affinity constant of 3.43nM as determined in BLI assay(QC tested).
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| 説明 |
FCGR4 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 22.4 kDa and the accession number is A0A0B4J1G0. |
| Species |
Mouse
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| Expression Host |
HEK293 Cells
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| Tag |
His
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| Accession Number |
A0A0B4J1G0
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| 別名 |
Fcgr3a, FcgammaRIV, Fcrl3, Fc receptor, IgG, low affinity IV, 4833442P21Rik, FcγRIV, CD16-2, FcgRIV
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| Construction |
The amino acid sequence corronding to Met 1-Gln 203 of Mouse FCGR4 (NP_653142.2) was fused with a polyhistidine tag at the C-terminus.
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| Protein Purity |
> 95 % as determined by SDS-PAGE
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| 分子量 |
22.4 kDa (predicted)
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| Endotoxin |
< 1.0 EU/μg of the protein as determined by the LAL method.
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| Formulation |
Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
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| Reconstitution |
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
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| Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
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| Shipping |
In general, Lyophilized powders are shipping with blue ice.
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