FCGRT & B2M Heterodimer Protein, Human, Recombinant (His), Biotinylated is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 43.5 kDa and the accession number is P55899&P61769.
| 生物学的情報 |
Using the Octet RED System, the affinity constant (Kd) of FCGRT & B2M Heterodimer Protein, Human, Recombinant (His Tag), Biotinylated bound to IgG1 Antibody was 4.6 nM.
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| 説明 |
FCGRT & B2M Heterodimer Protein, Human, Recombinant (His), Biotinylated is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 43.5 kDa and the accession number is P55899&P61769. |
| Species |
Human
|
| Expression Host |
HEK293 Cells
|
| Tag |
His
|
| Accession Number |
P55899&P61769
|
| 別名 |
Fc fragment of IgG, receptor, transporter, α, FCRN, α-chain, Fc fragment of IgG, receptor, transporter, alpha, alpha-chain
|
| Construction |
The extracellular domain (Met1-Ser297) of Human FCGRT (P55899) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; The Human B2M (P61769) (Met1-Met119) constructed the plasmid 2. The two plasmids were co-expressed and the FCGRT / B2M heterodimer was purified. The purified protein was biotinylated in vitro.
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| Protein Purity |
> 95 % as determined by SDS-PAGE
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| 分子量 |
43.5 kDa (predicted)
|
| Endotoxin |
< 1.0 EU/μg of the protein as determined by the LAL method.
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| Formulation |
Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
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| Reconstitution |
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
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| Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
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| Shipping |
In general, Lyophilized powders are shipping with blue ice.
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