FCGRT & B2M Heterodimer Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 43.5 kDa and the accession number is P55899-1&P61769.
| Species |
Human
|
| Expression Host |
HEK293
|
| Tag |
His
|
| Accession Number |
P55899-1&P61769
|
| 別名 |
Fc fragment of IgG, receptor, transporter, alpha, alpha-chain, α-chain, Fc fragment of IgG, receptor, transporter, α, FCRN
|
| Construction |
A DNA sequence encoding the extracellular domain (Met1-Ser297) of human FCGRT (P55899-1) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the human B2M (P61769) (Met1-Met119) constructed the plasmid 2. The two plasmids were co-expressed and the FCGRT / B2M heterodimer was purified.
|
| Protein Purity |
> 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC
|
| 分子量 |
43.5 kDa (predicted)
|
| Endotoxin |
< 1.0 EU per μg of the protein as determined by the LAL method.
|
| Formulation |
Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
|
| Reconstitution |
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
|
| Stability & Storage |
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping |
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
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