FGF-1 Protein, Human, Recombinant

FGF-1 Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 16 kDa and the accession number is P05230-1.

FGF-1 Protein, Human, Recombinant

パッケージサイズ	在庫状況	単価(税別)
10 μg	在庫あり	￥ 13,000
20 μg	約5 days	￥ 17,500
50 μg	約5 days	￥ 26,500
100 μg	約5 days	￥ 36,000

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生物学的情報	Measured in a cell proliferation assay using Balb/c 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.15-0.75 ng/mL.
説明	FGF-1 Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 16 kDa and the accession number is P05230-1.

Species	Human
Expression Host	E. coli
Tag	Tag Free
Accession Number	P05230-1
別名	AFGF, ECGF-beta, ECGFB, fibroblast growth factor 1 (acidic), GLIO703, ECGF, ECGF-β, HBGF-1, FGF-alpha, FGFA, FGF-α, ECGFA, HBGF1, FGF-1
Construction	A DNA sequence encoding the mature form of human FGF acidic (AAA79245.1) (Phe 16-Asp 155) was expressed, with an additional Met at the N-terminus.
Protein Purity	> 95 % as determined by SDS-PAGE
分子量	16 kDa (predicted)

Endotoxin	Please contact us for more information.
Formulation	Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution	A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Stability & Storage	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	aFGF, also known as FGF1 and HBGF-1, is a member of the fibroblast growth factor family. The biological activity of aFGF protein is exerted through binding to four high affinity cell surface receptors (FGFR1–4), which results in receptor dimerization and transphosphorylation in the tyrosine kinase domain. aFGF protein shows a wide range of endocrine-like activities. As a multiple function growth factor, this protein is involved in embryo development and tissue repair. Additionally, this protein is considered to function in several important physiological and pathological processes, such as embryonic development, morphogenesis, angiogenesis, wound healing and atheromatosis, carcinogenesis, development, and invasion of cancer.References

参考文献

1. Jaye M., et al.,(1986), Human endothelial cell growth factor: cloning, nucleotide sequence, and chromosome localization. Science 233:541-545. 2. Mergia A., et al., (1989), Structural analysis of the gene for human acidic fibroblast growth factor.Biochem. Biophys. Res. Commun. 164:1121-1129. 3. Wang W.P., et al.,(1989), Cloning of the gene coding for human class 1 heparin-binding growth factor and its expression in fetal tissues.Mol. Cell. Biol. 9:2387-2395.

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FGF-1 Protein, Human, Recombinant

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