FGFR1 alpha (IIIc) Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 42 kDa and the accession number is P11362-7.
| 説明 |
FGFR1 alpha (IIIc) Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 42 kDa and the accession number is P11362-7. |
| Species |
Human
|
| Expression Host |
HEK293
|
| Tag |
C-His-Avi
|
| Accession Number |
P11362-7
|
| 別名 |
FGF R1a, FGFR1 α, FGFR1 alpha, FGFR1 α (IIIc)
|
| Construction |
Recombinant Biotinylated Human FGFR1 alpha (IIIc) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Arg22-Glu374.[Accession |P11362-7]
|
| Protein Purity |
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
|
| 分子量 |
The protein has a predicted MW of 42 kDa. Due to glycosylation, the protein migrates to 68-80 kDa based on Tris-BisPAGE result.
|
| Endotoxin |
Less than 1EU per μg by the LAL method.
|
| Formulation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
|
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
|
| Stability & Storage |
-20 to -80°C for 12 months as supplied from date of receipt. -20 to -80°C for 3-6 months in unopened state after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
|
| Shipping |
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
|
| Research Background |
Fibroblast growth factors (FGFs) comprise a family of at least eighteen structurally related proteins that are involved in a multitude of physiological and pathological cellular processes, including cell growth, differentiation, angiogenesis, wound healing and tumorgenesis. The biological activities of the FGFs are mediated by a family of type I transmembrane tyrosine kinases which undergo dimerization and autophosphorylation after ligand binding.FGFR1 is tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of embryonic development, cell proliferation, differentiation and migration. Required for normal mesoderm patterning and correct axial organization during embryonic development, normal skeletogenesis and normal development of the gonadotropin-releasing hormone (GnRH) neuronal system.
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参考文献
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