FGFR2 alpha (IIIc) Protein, Human, Recombinant (His & Avi), Biotinylated

FGFR2 alpha (IIIc) Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 42.5 kDa and the accession number is P21802-1.

FGFR2 alpha (IIIc) Protein, Human, Recombinant (His & Avi), Biotinylated

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 187,000
500 μg	約5 days	￥ 746,000
1 mg	約5 days	￥ 1,248,500

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説明	FGFR2 alpha (IIIc) Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 42.5 kDa and the accession number is P21802-1.

Species	Human
Expression Host	HEK293 Cells
Tag	C-His-Avi
Accession Number	P21802-1
別名	BFR-1, FGF R2a, TK25, TK14, BEK, BBDS, JWS, FGFR2 α (IIIc), KGFR, ECT1, CD332, FGFR2 alpha, CEK3, K-SAM, FGFR2 α, CFD1, FLJ98662
Construction	Arg22-Glu377
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
分子量	42.5 kDa (predicted). Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.

Endotoxin	< 1 EU/μg by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Four distinct genes encoding closely related FGF receptors, FGF R1 - 4, are known. All four genes for FGF Rs encode proteins with an N-terminal signal peptide, three immunoglobulin (Ig)-like domains, an acid-box region containing a run of acidic residues between the IgI and IgII domains, a transmembrane domain and the split tyrosine-kinase domain. Multiple forms of FGF R1 - 3 are generated by alternative splicing of the mRNAs. A frequent splicing event involving FGF R1 and 2 results in receptors containing all three Ig domains, referred to as the alpha isoform, or only IgII and IgIII, referred to as the beta isoform.

参考文献

1. D'Amici S, et al. TNFα modulates Fibroblast Growth Factor Receptor 2 gene expression through the pRB/E2F1 pathway: identification of a non-canonical E2F binding motif. PLoS One. 2013 Apr 16;8(4):e6149doi: 10.1371/journal.pone.006149PMID: 23613863; PMCID: PMC3629046.

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FGFR2 alpha (IIIc) Protein, Human, Recombinant (His & Avi), Biotinylated

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